当归多糖在体外抑制HCMV感染所致巨核系细胞凋亡中的作用探讨

本研究探讨当归多糖（angelica polysaccharide，APS）在体外抑制HCMV感染致人巨核系细胞凋亡中的作用。HCMV AD169体外感染巨核系细胞CHRF-288-11，在病毒感染后第3天向试验体系中加入APS。用PCR扩增检测HCMV DNA，用形态学、DNA片段化、细胞表面标志检测APS对HCMV感染所致巨核系细胞凋亡的影响。结果表明：APS在一定程度上能抑制HCMV体外感染诱导的巨核系细胞CHRF-288-11凋亡，且呈剂量依赖性。感染的巨核系细胞CHRF-288-11中有hCMV IEA的表达，形态学和DNA片段化分析证实了细胞凋亡的存在，Annexin V／PI双染流式细胞仪分析显示受感染的CHRF细胞中随着加入APS剂量的减少，凋亡率呈上升趋势。结论：HC—MVAD169株在体外可直接感染巨核系细胞并降低其存活率；HCMVAD169株在体外感染巨核系细胞后可诱导其加重凋亡，凋亡率与时间呈正相关。在HCMVAD169株体外感染巨核系细胞后，向培养细胞中加入APS，可以提高受染细胞的存活率，表明APS对HCMV感染的巨核系细胞有一定的保护作用；APS在一定程度上能抑制HCMV体外感染诱导的巨核系细胞凋亡，且呈剂量依赖性。

In Vitro Suppressive Effect of Angelica Polysaccharide on Human Cytomegalovirus-induced Apoptosis via Direct Infection in CHRF-288-11 Cells

The objective of study was to investigate the in vitro suppressive effect of angelica polysaccharide （APS） on human cytomegalovirus-induced apoptosis via direct infection in CHRF-288-11 cells. HCMV AD169 directly infected CHRF-288-11 were cultured in vitro, APS in different doses were added on day 3 after the infection of virus. Cells of every group were collected at different time points. HCMV DNA of cells were detected by using polymerase chain reaction and the apoptotic cells were examined by using Hoechest staining, MTT assay, DNA fragmentation assay and flow cytomertry. The results showed that the APS to some extent inhibited the apoptosis of CHRF cells infected by HCMV in vitro in a dose-dependent manner. The expression of HCMV IEA in CHRF-288-11 cells was found by PCR amplifica- tion. Morphology observation, flow cytomertry assay and DNA fragmentation assay revealed the existence of apoptosis. With the dose decrease of APS added to the infected CHRF cells, the percentage of apoptotic cells increased. It is concluded that the HCMV AD169 can infect CHRF cells directedly in vitro and decrease cell viability. HCMV AD169 infection increases the apoptosis of CHRF cells in time-dependent manner. When APS was added to the CHRF cells infected by HCMV AD169 in vitro, the viability of CHRF cells increase, which indicated that APS to some extent protects the CHRF cells infected by HCMV. APS suppresses the cytomegalovirus -induced apoptosis in CHRF cells directly infected in vitro in dose-dependent manner.