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| 用聚合酶链反应技术检测膀胱癌病毒基因 | |
| 引用本文： | 王宝成,杨道理,孙晓明,齐法莲摘要. 用聚合酶链反应技术检测膀胱癌病毒基因[J]. 解放军医学杂志, 1994, 0(3) |
| 作者姓名： | 王宝成 杨道理 孙晓明 齐法莲摘要 |
| 作者单位： | 济南军区总医院 |
| 摘 要： | 用聚合酶链反应（ＰＣＲ）技术对６８例膀胱癌及１５例正常膀胱粘膜中第１６、１８型人乳头瘤病毒（ＨＰＶ＿１６、１８）ＤＮＡ进行了检测。结果表明，新鲜及石蜡包埋的癌组织ＨＰＶ＿１６ＤＮＡ的阳性率分别为５３．８％及６６．７％，ＨＰＶ＿１８ＤＮＡ的阳性率为４．４％。ＨＰＶ＿１６ＤＮＡ在癌组织中出现与恶性度及浸润深度有一定关系。正常粘膜有少量ＨＰＶ＿１６ＤＮＡ存在。从正常粘膜到癌组织，ＨＰＶ＿１６ＤＮＡ检出率逐渐增高，反映出其存在较为广泛及在癌变中的作用。 |
| 关 键 词： | 聚合酶链反应，病毒基因，膀胱肿瘤 |
DETECTION OF VIRUS GENES IN CARCINOMA OF BLADDER BY POLYMERASE CHAIN REACTION |
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| Wang Baocheng,Yang Daoli,Sun Xiaoming,et al.. DETECTION OF VIRUS GENES IN CARCINOMA OF BLADDER BY POLYMERASE CHAIN REACTION[J]. Medical Journal of Chinese People's Liberation Army, 1994, 0(3) | |
| Authors: | Wang Baocheng Yang Daoli Sun Xiaoming et al. |
| Affiliation: | Wang Baocheng,Yang Daoli,Sun Xiaoming,et al. General Hospital of Jinan Command,Jinan 250031 |
| Abstract: | Determination of human papilloma virus type l6 and l8 (HPV16.18)in 68 cases car-cinoma of bladder and l5 cases normal bladder mucous membrane with polymerase chain reaction(PCR was reported. The resuIts showed that the detaction rates of HPV16 DNA in fresh andparaffin embedded tissue were 53.8%and 66.7%, respectively, while the positive rate of HPV18DNA was 4.4%. The investigation also revealed that the appearance of HPV16 DNA in paracarcinoma tissue was related to the degree of malignancy and the depth of infiItration. Low levels of HPV16DNA were also detected in normal bladder mucous membrane, which reflected the widespread exis-tence of HPV16 DNA and its potential role in carcinomas. |
| Keywords: | Polymerase chain reaction Virus gene Carcinoma of bladder |
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