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The determination of the complement components C1q, C4 and C3 in serum and cerebrospinal fluid by radioimmunoassay |
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| Authors: | B. C. G. Dujardin P. C. Driedijk A. F. M. Roijers T. A. Out |
| Affiliation: | a Central Laboratory of the Netherlands Red Cross Blood Transfusion Service and Laboratory for Experimental and Clinical Immunology of the University of Amsterdam, Amsterdam, The Netherlands 1 Clinical Chemistry Laboratories, Medical Center Alkmaar, Alkmaar, The Netherlands |
| Abstract: | Non-competitive 2-site radioimmunoassays (RIA) for the determination of the complement proteins C1q, C4 and C3 in cerebrospinal fluid (CSF) are described. The quantitative results of the RIAs were the same as those obtained by other assay methods: radial immunodiffusion and turbidimetry and, in the case of C4, the haemolytic assay. The concentrations of the complement proteins in paired CSF and serum samples from a group of 60 patients were measured, as well as those of albumin and IgG. The ratios (concentration in CSF)/(concentration in serum) of the complement proteins correlated poorly with that of albumin. In contrast, the ratio of IgG was significantly correlated with that of albumin. The ratios of the complement proteins were higher than might be expected on the basis of their molecular masses. This suggests that these proteins may be synthesized within the normal central nervous system. |
| Keywords: | complement C1q, C3, C4 radioimmunoassay cerebrospinal fluid hemolytic assay blood CSF barrier reference values |
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