儿童支气管哮喘患儿外周血DC细胞数量和功能的分析研究

目的 观察支气管哮喘患儿外周血树突状细胞(DC)数量和亚型细胞的变化,研究DC细胞在支气管哮喘发病中的作用及可能的发病机理.方法 38例支气管哮喘患儿作为哮喘组,将哮喘组患儿随机分为控制组(21例)及新诊断组(17例),选取17例同期本院保健科体检健康儿童作为对照组,利用流式细胞技术先分选DCs细胞,后分选出亚型细胞:...

Analysis of the number and function of peripheral blood DC cells in children with bronchial asthma

Objective To observe the changes in the number and subtypes of peripheral blood dendritic cells(DC)in patients with bronchial asthma,and study the role of DC cells in the pathogenesis of bronchial asthma and the possible pathogenesis.Methods There were 38 children with bronchial asthma selected as the asthma group.The children in the asthma group were randomly divided into control group(21 cases)and newlydiagnosed group(17 cases).17 healthy children who had physical examinations in the health department of the hospital during the same period were selected as the reference group.DCs were sorted by flow cytometry,and then the subtypes of DCs were selected:plasma like DC(p DC)and conventional DC(c DC).The peripheral blood c DC number,DC function indicators[interleukin(IL)-12,eosinophil ratio(EOS%)and immunoglobulin E(IgE)]were analyzed and compared among the three groups.Results The number of p DC(14.19±6.89)×10⁶/L of newly-diagnosed group was higher than(9.70±4.90)×10⁶/L of the control group and(8.52±4.18)×10⁶/L of the reference group,and the difference was statistically significant(P<0.05).There was no statistically significant difference between the control group and reference group(P>0.05).The number of c DC(3.85±1.32)×10⁶/L of newly-diagnosed group had no statistically significant difference compared with(4.16±1.51)×10⁶/L of control group and(3.67±1.85)×10⁶/L of reference group(P>0.05).The IL-12(28.65±10.20)pg/ml of newly-diagnosed group was lower than(31.23±7.93)pg/ml of the control group and(41.68±11.53)pg/ml of reference group,and EOS%(12.3±4.6)%and Ig E(863±56)IU/ml were higher than the control group[(8.5±3.2)%and(231±38)IU/ml]and reference group[(2.3±1.2)%and(32±16)IU/ml].The IL-12 of the control group was lower than the reference group,and EOS%and Ig E were higher than the control group,and the difference was statistically significant(P<0.05).Conclusion p DC/c DC subtype drift causes Th1/Th2 immune dysfunction,and the activation of immune inflammatory response by p DC characteristics and dysfunction may play an important role in the occurrence and development of bronchial asthma.