乳腺癌肌上皮细胞的免疫组织化学观察

作者应用免疫组化技术对50例乳腺癌、20例乳腺良性病变以S—100蛋白和癌胚抗原(CEA)作PAP染色对肌上皮细胞进行了观察。结果表明:1.所有乳腺良性病变S—100标记显示肌上皮细胞均有不同程度的增生,而各型乳腺癌的癌巢、管状结构周边均无S—100阳性的细胞。因此可确认,肌上皮细胞的增生或消失对判断乳腺良恶性病变有着重要意义;2.50例乳腺癌的癌细胞S—100标记,有23例呈不同程度的阳性(46％);3.S—100标记显示阳性的细胞,在H·E切片上,细胞核有异型性,作者认为肌上皮细胞参与了癌瘤的生成;4.50例乳腺癌CEA有不同程度的表达者占90％(45/50),S—100阳性表达占46％(23/50)。我们同意Biggs和McDivitt认为乳腺癌来自具有双向分化能力的干细胞这一推论。

IMMUNOHISTOCHEMICAL STUDY ON MYOEPITHELIUM CELLS IN BREAST CARCINOMA

The expression of S-100 protein (S-100) and carcinoembryonal antigen (CEA)in myoepithelium cells of 50 breast carcinoma and 20 benign lesion of breast were studied using PAP method (some cases using Immuno-Gold, -silver double staining). The results indicatd:1. Myoepithelium cells proliferated in varying degrees in all benign lesions of breast, showed S-100 positivity. However, there was no any S-100 positive cells in breast carcinoma nest and cells around tubular structure. So, we believe that the proliferation or disappearance of myoepithelium cells has significance for differentiating the benign and malignamt lesion of breast. 2. 23 among 50 (46%).breast carcinoma patients expressed S-100 protein in varying degrees. 3. The Z-100 positive cells showed nuclear atypia in HE stained slides. It was thought that myoepithelium cells participated in the neoplastic transformation. 4. CEA was expressed in 45/50 cases (90%), and S-100 was positive in 23/50 cases. We agree with the hypothesis that breast carcinoma originated from the stem cells with two-way bifferentiation potential.