促进内皮细胞在小口径人工血管表面的黏附方法探讨

人工血管内皮化是解决人工血管移植体内后血栓形成和提高远期通畅率的最有前途的途径之一.为了使内皮细胞在小口径人工血管表面形成一层完整的覆盖并减少血栓形成,一般采用对人工血管做黏附基底预处理、在生物反应器中对人工血管进行三维流动培养、对内皮细胞进行基因修饰以及改变内皮细胞的带电性质等方法.本文对此方面的研究进展进行了综述,初步探讨了促进内皮细胞在小口径人工血管表面黏附的方法.

Research on Endothelialization of Artificial Vascular Grafts

Endothelialization of artificial vascular graft is considered as one of the most promising methods to improve its antithrombotic ability and long-term patency. Endothelialization of artificial vascular graft includes harvesting endothelial cells,choosing some materials with better compliance and seeding endothelial cells. The methods such as immobilization of extracellular matrix protein and growth factor to substrates,stimulation of chronic in vitro shear stress for endothelial cell retention on artificial vascular graft in bioactor,genetic modificatioin of ECs, and changes of electric charge of ECs are used to increase the adherence ability of endothelial cells.This paper reviews the process of endothelialization of artificial vascular graft and makes brief comments on the methods of endothelialization of artificial vascular graft.