ras、c—erbB—2、P^53基因与金葡菌L型感染在实验性小鼠肿瘤中的作用

目的 探讨癌基因ras、c-erbB-2和抑癌基因P^53与金葡菌L型感染在C57小鼠致瘤中的作用和意义。方法 应用免疫组化及核酸原位杂交对L型感染后诱发的10只小鼠肿瘤的13只小鼠癌前病变组织进行ras、c-erbB-2及P^53蛋白产物及基因的检测。结果 小鼠肿中P^21、c-erbB-2、P^53的蛋白表达阳性率分别为60％、70％、60％；癌前病变15．38％、15．38％、38．46％。原位核酸杂交ras、c-erbB-2、P^53、mRNA表达在小鼠肿瘤中分别为50％、70％、50％；癌前病变中为15．38％、0％、38．46％。结论 癌基因ras、c-erbB-2及抑癌基因P^53在小鼠肿瘤中有不同程度异常表达，L型极有可能成为诱癌因素之一，它们有协同致癌作用。

THE SIGNIFICANCE AND ROLE OF RAS,C-ERBB-2,P~(53) GENE EXPRESSION AND INFECTED WITH BACTERIAL L-FORMS IN TUMOR OF C_(57) MICE

Aim To evaluate the signficance and role between ras,c-erbB-2 onc-gene and P 53 tumor suppressor gene expression infected with bacterial L-forms in tumor of C 57 mice.Methods 10 cases of tumor and 13 cases precancerous lesion in mice infected with bacterialL-forms were studied for ras,c-erbB-2,and P 53 expression of protein and mRNA with immunohistochemistry and hybridization in situ.Results The positive rate of P 21 (ras),c-erbB-2 and P 53 protein expression were 60%(6/10),70%(7/10) and 60%(6/10) in 10 cases of tumor mice,and were 15.38%(2/13),15.38%(2/13) and 38.46%(5/13) in 13 cases of precancerous lesion mice.The positive rate of ras,c-erbB-2 and P 53 mRNA expression were 50%(5/10),70%(7/10) and 50%(5/10) in 10 cases of tumor mice,and were 15.38%(2/13),0%(0/13) and 38.46%(5/13) in 13 cases of precancerous lesion mice respectively.Conclusion The results demonstrate that the abnormalities of ras,cerbB-2 and P 53 protein and mRNA expression,infection bacterial L-forms may be one of causes of tumor.They may be related to the pathogenesis and development of tumor of mice.