| 高效液相色谱法测定金银花连翘提取物(注射用)中连翘苷的含量 |
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| 引用本文： | 仉晶,金智利. 高效液相色谱法测定金银花连翘提取物(注射用)中连翘苷的含量[J]. 黑龙江医药, 2011, 24(1): 17-19 |
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| 作者姓名： | 仉晶 金智利 |
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| 作者单位： | 哈药集团三精制药股份有限公司 |
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| 摘 要： | 目的:建立高效液相色谱法测定金银花连翘提取物(注射用)中连翘苷的含量.方法:采用迪马(钻石)C18(250mm ×4.6mm,5μm)色谱柱,流动相为甲醇-水-冰乙酸(20:80:1),流速1.0ml/min,检测波长:324nm.结果:金银花连翘提取物中连翘苷能达到有效分离,在10.04μg/mlg～150.60μg...
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| 关 键 词： | 高效液相色谱法 连翘苷 含量测定 |
Determination of Phillyrin Content in Honeysuckle and Fersythia Extracts(Injection Use)by HPLC |
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| Affiliation: | Zhang Jing,et alSanjing Pharmaceutical Co.ltd,Harbin Pharm.Group |
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| Abstract: | Objective：To establish a HPLC method for determination of Phillyrin Content in Honeysuckle and Forsythia extracts（Injection Use）.Methods：Diamonsil C18 high performance liquid chromatography column（250mm×4.6mm,5μm）produced by Dikma technologies was used.The mobile phase consisted of Methyl alcohol-water-lglacial acetic acid（20：80：1）,and the flow rate was 1.0ml/min.The detection wavelength was 324nm.Results：Phillyrin in Honeysukle and Forsythia extracts could be separated well.The linearity of the dehydroandrographolide Succinate curve was well correlated within the range of 10.04-150.60μg/ml.The instrument precision RSD was 0.03%;The method precision RSD was 0.96%.Conclusion：The methods appeared to be simple,accurate and reproducible.It can be used to control the quality of Honeysuckle and Forsythia Extracts. |
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| Keywords: | HPLC Phillyrin assay |
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