MPTP对小鼠不同脑区神经递质代谢酶基因表达的影响

1-甲基 -4 -苯基 -1,2 ,3 ,6-四氢吡啶 (MPTP)能够诱发黑质纹状体系统多巴胺能神经元损伤并产生与帕金森病类似的症状。本实验通过 MPTP注射诱导 C5 7BL小鼠神经元损伤 ,利用逆转录 PCR方法检测酪氨酸羟化酶 (TH)、单胺氧化酶 B(MAO-B)以及胆碱乙酰基转移酶 (Ch AT)三种神经递质代谢相关基因的表达变化。结果发现 ,在小鼠黑质与纹状体中 ,MPTP导致 TH基因表达显著降低 ,而 MAO-B与 Ch AT基因的表达变化在黑质与纹状体则有所不同 ,在黑质 MPTP导致 MAO-B与 Ch AT基因表达的上升 ,在纹状体则略有下降。

GENE EXPRESSIONS OF NEUROTRANSMITTER METABOLISM RELATED ENZYMES IN SUBSTANTIA NIGRA AND STRIATUM OF MPTP-TREATED MICE

Administration of 1 methyl 4 phenyl 1,2,3,6 tetrahydropyridine (MPTP) to mammals causes damage to the nigrostriatal dopaminergic pathway similar to that observed in Parkinson's disease. The gene expressions of tyrosine hydroxylase (TH), monoamine oxidase (MAO B) and choline acetyltransferase (ChAT) in substantia nigra and striatum of MPTP treated C57BL mice were detected by reverse transcriptional polymerase chain reaction (RT PCR). It was found that the expression of TH gene was decreased in the substantia nigra and striatum of MPTP treated mice, the expressions of MAO B and ChAT genes were increased in substantia nigra and showed no significant difference in striatum.