牛血浆纤维粘连蛋白对培养的鼠牙乳头组织的影响

目的观察牛血浆纤维粘连蛋白（ｆｉｂｒｏｎｅｃｔｉｎ，ＦＮ）对牙乳头组织及细胞的作用。方法对大鼠牙乳头和牙胚组织进行器官培养，实验组将微孔滤膜用８０ｍｇ／ＬＦＮ液浸润２ｈ，然后将牙乳头组织置薄膜上培养３、６天，同时设对照组，牙胚培养３、６、９天，常规组织学染色。结果牙胚在体外可有成牙本质细胞分化和前期牙本质的形成。实验组于牙乳头组织和滤膜交界处出现极化细胞，而对照组则未出现上述现象。结论ＦＮ参与了成牙本质细胞的极化过程。

Effect of bovine plasma fibronectin on rat papilla cells in vitro

Objective To observe the effect of exogenous fibronectin (FN) on the rat dental papilla tissues and cells.Methods Tooth germs of first mandibular molars obtained from 17 day old rat (SD) embryos were cultured for 3,6,9 days on millipore filters in DMEM supplemented with 10% FCS, 50mg/L Vitamin c. The dental papilla were isolated by trypsin treatment of homologous tooth germs and cultured on uncoated (control) and FN coated millipore filters for 3,6 days, bovine plasma FN deposited on millpore filters by treated for 2 hours with 80mg/L FN.Results As shown by histological and cytological data, some mesenchymal cells differentiated into odontoblasts and deposited predentine in cultured tooth germs. In cultured dental papillas, polarized functional cells were observed in FN coated group, odontoblast like cells never differentiated in control cultures.Conclusion FN may be involved in odontoblast polarization.