消毒剂对两种动物阴道黏膜刺激试验结果比较研究

为比较两种动物阴道黏膜对不同受试物刺激敏感性,采用大鼠和家兔阴道黏膜刺激性试验观察黏膜消毒剂及卫生用品刺激反应及病理变化。结果,黏膜消毒剂对家兔与大鼠阴道黏膜刺激试验阳性反应检出率分别为56.3%和15.8%。黏膜消毒剂对大鼠阴道黏膜刺激反应强度均为无刺激性,对家兔阴道黏膜有极轻刺激性者占37.5%;一次性卫生用品对家兔或大鼠阴道黏膜刺激强度分级均为无刺激性。40种产品对动物阴道黏膜组织病理检查病理改变检出率为97.5%;主要病理改变有黏膜下水肿、血管充血及炎细胞浸润等。结论,黏膜消毒剂对大鼠阴道黏膜刺激反应率明显低于家兔的反应率;细胞水平病理组织学检查对评价受试物阴道黏膜组织损伤的程度比较敏感,对受试物的刺激强度易于分级。

COMPARISON OF RESULTS OF TESTS FOR IRRITATING EFFECTS OF DISINFECTANTS ON VAGINAL MUCOSA OF TWO KINDS OF ANIMALS

In order to compare the sensitivity of vaginal mucosa of two kinds of animals to irritation by different test substances,rat and rabbit vaginal mucosa irritation tests were used to observe the irritation reaction and pathological changes caused by mucosa disinfectants and sanitary products.Results: The rates of positive reaction in the tests for irritating effects of mucosa disinfectants on rabbit and rat vaginal mucosa were 56.3% and 15.8% respectively.The irritation reaction intensity of rat vaginal mucosa to mucosa disinfectants was no irritation,while the vagina disinfectants causing very mild irritation of rabbit vaginal mucosa accounted for 37.5%.The irritating intensity grades of the disposable sanitary products to either rabbit or rat vaginal mucosa were no irritation.The rate of pathological changes detected in the animal vaginal mucosa exposed to 40 kinds of products was 97.5%.The main pathological changes were submucosal edema,congestion and infiltration of inflammatory cells.Conclusions: The rate of irritation reaction of rat vaginal mucosa exposed to mucosa disinfectant was significantly lower than that of rabbit vaginal mucosa.Pathological examination at cell level is relatively sensitive for evaluating the degree of vaginal mucosa damage caused by test substance and facilitates grading of irritating intensity of the test substance.