鼠血管内皮生长因子及其抗体对内皮细胞增生影响的研究

目的 研究探讨血管内皮生长因子（VEGF）及其抗体对内皮细胞增生的影响。方法 采用体外培养人脐静脉内皮细胞（HUVEC），用^3H－TaR掺入检测技术分别检测鼠血管内皮生长因子（mVEGF）及其抗体和Ⅱ型胶原诱导的关节炎（CIA）小鼠关节组织提取液对HUVEC增生影响。结果 抗VEGF中和抗体抑制在mVEGF刺激下引起的HUVEC增生及在CIA小鼠关节组织提取液作用下引起的HUVEC增生，抑制率分别为72．2％和69．9％。结论 mVEGF特异性作用于血管内皮增生；CIA形成的早期关节组织内可能产生大量的VEGF，并以较强的生物活性形式存在，能被抗VEGF中和抗体抑制。

Effects of murine vascular endothelial growth factor (mVEGF) and anti- vascular endothelial growth factor (anti-VEGF) antibady on human umbilicus vein endothelial cell (HUVEC) proliferation

Objective To study on effects of mVEGF and anti VEGF antibody during cultured HUVEC proliferation in vitro .Methods Endothelial cell proliferation was assayed using human umbilical vein endothelial cells stimulated with mVEGF and with CIA joint extracts and was used 3 H TaR incorporation.Results The anti VEGF neutralizing antibody can inhibit the proliferation of HUVEC stimulated with mVEGF and with CIA joint extracts,whose suppression percents were 72 2% and 69 9%,respectively.Conclusion mVEGF specifically promotes the growth of vascular endothelial cells.During early stage of CIA development,expression of VEGF in the joint increases and VEGF is expressed biologically active and can be inhibited by anti VEGF neutralizing antibody.