口底鳞状细胞癌形态计量学研究的临床意义

目的　探讨细胞形状因子(FC)和平均光密度参数在口底鳞癌诊断中的意义。方法　28例口底鳞癌石蜡标本切片免疫组化(SABC)染色,应用图像分析仪测定原发灶和颈淋巴结细胞胞核形状因子(FC)和平均光密度参数。结果　经CK10和CK14单克隆抗体标记后,淋巴结有无转移或病理分级不同患者的原发灶细胞胞核中FC和平均光密度参数有显著性差异(P<0·05或<0·001),而临床分期不同的患者,其差异无显著性(P>0·05);颈淋巴结中转移阳性和阴性间的FC和平均光密度有显著性差异(P<0·05或<0·001)。结论　CK10和CK14表达中细胞的FC 和平均光密度参数可作为评估口底鳞癌浸润、转移潜能的临床参考指标。

Clinic Significance of Morphometric Study on Squamous Cell Carcinoma of Floor of Mouth

Objective The purpose of this study was to investigate the role of cell morphometric factor (FC) and the average density of light in squamous cell carcinomas at the floor of mouth by immunohistochemical method (SABC).Methods The slices made from the paraffin specimens of 28 patients with squamous cell carcinomas at the floor of mouth were stained by the immunohistochemical technique with monoclonal antibody CK-{10} and CK-{14}.In these stained slices the FC parameters and the average density of light were measured by using an image analysis system.Results In primitive tumors, the FC parameters and the average density of light were significantly different between positive and negative lymph nodes metastasis or between different pathological grades(P<0.05 or <0.001).The significance was not existed among clinical stages(P>0.05). The difference of FC expressed in positive and negative lymph nodes metastasis were significant(P<0.05 or <0.001).Conclusion The cell morphometric factor (FC) parameters and the average density of light of CK-{10} and CK-{14} could be used as a biological guideline to evaluate the clinically infiltrative and metastasic potential of squamous cell carcinoma at the floor of mouth.