蚯蚓纤溶酶研究概述

对具有纤溶活性的物质研究已有多年的发展,但是血栓性疾病仍然是致人死亡或身体机能障碍的主要原因之一。从具有特殊生理功能的生物体中获取活性物质近来受到广泛关注,在蚯蚓消化腺的分泌物中纯化得到一组同工酶,表现出良好的溶栓效果,并被命名为蚯蚓纤溶酶（earthworm fibrinolytic enzyme,EFE）。本文在多层次上对蚯蚓纤溶酶作一综述,内容包括：蚯蚓纤溶酶的纯化、克隆表达,并详细介绍该酶结构与功能之间的关系以及临床应用等。

An Overview of the Fibrinolytic Enzyme from Earthworm

Although many fibrinolytic agents have been developed and used for clinical purposes,thromboembolic diseases remain the leading cause of adult morbidity and mortality in the world.Developing effective agents for clinical application from various unusual animal species has received more attention in recent years.A group of fibrinolytic enzymes named earthworm fibrinolytic enzyme（EFE） and secreted by the alimentary tract of earthworm,have exhibited excellent potential in the clinical treatment of blood clotting diseases.It is therefore useful to build up some correlations with the data available in order to better explore the molecular and cellular mechanism of EFE action in the treatment of diseases.The aspects discussed include：the purification,characterization,gene cloning and expression,structure and function as well as clinical application of the EFEs.This review focuses on recent advances in molecular and cellular mechanisms of EFE action and the structure activity relationship.