苦参碱对小鼠急性酒精性肝损伤的保护作用研究

目的：研究苦参碱对小鼠急性酒精性肝损伤的保护作用并初步探讨其机制。方法：采用♂昆明小鼠随机分为正常对照组、模型组、阳性对照（硫普罗宁30mg/kg）组、苦参碱高、中、低剂量（80mg/kg、40mg/kg和20mg/kg）组,分别灌胃给药6天,于第6天末次给药2h后除正常对照组外,其余各组一次性灌胃给予50%乙醇（5g/kg）。乙醇处理12h后测定血清谷草转氨酶（AST）、谷丙转氨酶（ALT）的活性及肝脏组织匀浆丙二醛（MDA）、超氧化物歧化酶（SOD）和谷胱甘肽还原酶（GSH-px）的含量,并进行肝组织病理学切片检查及Ridit分析。结果：与模型组比较,苦参碱各剂量组血清AST、ALT活力和肝组织MDA含量显著降低（P〈0.05）;肝组织SOD、GSH-Px活力均升高（P〈0.05）;苦参碱高、中剂量组R值显著于模型组（P〈0.05）。结论：苦参碱对小鼠急性酒精性肝损伤具有保护作用,其保护作用可能与抗氧化应激相关。

Protective Effects of Matrine on Acute Ethanol-Induced Liver Injury in Mice

Objective：To investigate the protective effect of matrine on acule ethanol-induced liver injury in mice and its mecha- nism. Methods： Male Kunming mice were randomly divided into the control group, model group, positive control group （Tiopronin） and different concentration of matrine （80 mg/kg, 40 mg/kg and 20 mg/kg） and irrigation stomach respectively for 6 days. The mice were induced alcohol acute liver damage by ethanol （50%, 5 g/kg） at the last administration beside control group. After 12 hours ethanol treatment, the determination serum aspartate aminotransferase （AST） and alanine aminotransferase （ALT） ; MDA, SOD and GSH-px in liver tissue were detected. Pathological changes in liver 1issue were checked by the Ridit analysis method. Resuits： The determination of liver index, activity of serum AST and ALT and MDA in liver tissue homogenate were significantly reduced（P〈0.05）and the activity of SOD and OSH-px in liver tissue were increased（P〈0.05） in each dose of matrine. The value of R in matrine high and middle doses were decreased （P〈0.05）. Conchlsion： Matrine has a significant protection against acute ethanol induced hepatic injury, and the mechanism may be associated with anti-oxidative activities.