中药更年春(GNC)方对去势大鼠学习记忆能力及下丘脑神经递质的影响

 目的  观察中药更年春方(Gengnianchun,GNC)对去势大鼠学习记忆能力及下丘脑内神经递质的影响。方法  分为两批实验,均选用3月龄SD雌性大鼠,随机分为卵巢切除(ovariectomy,OVX)组、OVX+GNC组、OVX+戊酸雌二醇(estradiol valerate,E2)组、OVX组、Sham+GNC组和Sham组,第1批实验连续灌药3个月后经Morris水迷宫实验检测各组大鼠的学习记忆能力。第2批实验连续灌药1个月后采用高效液相色谱法检测各组大鼠下丘脑内神经递质去甲肾上腺素(NE)、多巴胺(DA)、5-羟色胺(5 HT)和5-羟吲哚乙酸(5-HIAA)的水平。结果  第1批实验中OVX1组与Sham1组比较,学习记忆能力明显减退(P＜0.05);OVX1+GNC组、OVX1+ E2组与OVX1组比较,学习记忆能力明显增强(P＜0.05);Sham1+GNC组与Sham1组比较,学习记忆能力明显减退(P＜0.05)。第2批实验中,OVX2组与Sham2组比较,NE、DA含量明显减少(P＜0.05),5-HT、5-HIAA含量明显增加(P＜0.05);OVX2+GNC组、OVX2+ E2组与OVX2组比较,NE、DA含量明显增加(P＜0.05),5-HT、5-HIAA含量明显减少(P＜0.05);Sham2+GNC组与Sham2组比较,NE、DA、5-HT、5-HIAA之间无明显差异(P＞0.05)。结论   GNC具有提高去势大鼠学习记忆能力的作用,并可维持下丘脑内神经递质的平衡,但未出现卵巢功能衰退则不宜补充GNC。

Effect of Gengnianchun (GNC) formula on the ability of learning and memory and neurotransmitter of hypothalamus in ovariectomized rats

Objective  To study the effect of Gengnianchun formula (GNC) on learing and neurotransmitter of hypothalamus in ovariectomized rats.Methods  The study included 2 experiments.Three-month old SD rats were randomized into OVX group,OVX+GNC group,OVX+ estradiol valerate (E2) group,sham operation (Sham) group and Sham+GNC group.The 5 experiment lasted for 3 months,and changes of learning memory ability were tested by Morris water maze.The 2nd one lasted for 1 month,and the level of neurotransmitter in hypothalamus (NE,DA,5-HT,5-HIAA) were measured by high-performance liquid chromatography (HPLC).Results  In the first experiment,ovariectomy,the learning and memory ability of OVX1 group and sham1+GNC group was markedly decreased compared with rats in Sham1 group (P＜0.05).While in OVX1+GNC and OVX1+E 2 group,the learning and memory was improved markedly compared with OVX1 group (P＜0.05).In the second experiment,NE and DA level in hypothalamus were decreased markedly,but 5-HT and 5-HIAA level in hypothalamus were markedly improved in OVX2 group than in sham2 group (P＜0.05).NE and DA level in hypothalamus were improved,and 5-HT and 5-HIAA level in hypothalamus were decreased in OVX2+GNC and OVX2+E 2 group compared with OVX2 group (P＜0.05).But no significant change was observed in neurotransmitters level in hypothalamus between Sham2 group and Sham2 +GNC group.Conclusions  GNC can improve the ability of learning and memory,and regulate the disorderly neurotransmitter in hypothalamus.But the rats without ovarian failure are not suit for GNC.