单纯及丙泊酚联合电休克处理对抑郁大鼠海马BDNF mRNA的影响

目的 观察单纯电休克及丙泊酚联合电休克治疗对抑郁大鼠电休克疗效及海马内脑源性神经营养因子(BDNF)mRNA的影响.方法 24只SD大鼠随机分为对照组(C组)、抑郁组(D组)、单纯电休克组(E组),丙泊酚联合电休克组(M组),每组6只.C组正常饲养,D、E、M组采用孤养加慢性不可预见性应激建立抑郁模型,建模后E组直接行电休克处理,M组在丙泊酚100 mg/kg腹腔注射麻醉下行电休克处理,以上各组处理隔天1次,共6次.以open-field法和Morris水迷宫法测大鼠行为学水平和学习记忆水平,实验完成后处死大鼠以实时定量PCR法检测海马BDNF mRNA表达.结果 电休克处理后E、M组大鼠水平计分、垂直计分分别为(35.8±12.0)分,(7.3±2.4)分,(39.6±14.0)分,(7.3±2.0)分],高于D组分别为(17.0±5.0)分,(3.3±1.0)分](P<0.05);E组逃避潜伏期长于D组、M组,日标象限游泳时间百分比低于D组、M组(P<0.05);E、M组大鼠海马BDNF mRNA相对表达量高于D组(P<0.05),2组间比较差异无统计学意义,D组低于C组(P

Effect of electroconvulsive therapy and propofol combined with electroconvulsive therapy on brain-derived neurotrophic factor mRNA in hippocampus of depressed rats

Objective To investigate the effect of propofol combined with electroconvulsive therapy on brain-derived neurotrophic factor in hippocampus of depressed rats. Methods 24 adult male SD rats weighing 200-250g were randomly divided into four groups(n= 6): control group (group C), depressed group (group D), electroconvulsive therapy group ( group E ) , propofol combined with electroconvulsive therapy group ( group M ) . The depression model of rat was produced by separation and chronic unpredictable mild stress. The anxiety-related behaviour and exploration of rats were tested in the open field. The expression of BDNF mRNA was measured by RT-PCR. Results Post-treatment, ambulation and rearing of open-field test were significantly increased in group E and group M compared with group D(P<0.05). Evasive latency of Morris water maze were significantly increased in group E compared with group D and M group E(35. 8 ±6. 8)s, group D(28.4 ±4.1)s, group M(23.8±7.0) s] (P<0.05). Swimming time percentage in platform quadrant of Morris water maze were significantly decreased in group E compared with group D and M(P<0.05). The expression of BDNF mRNA was significantly decreased in group D compared with the ones in group C (P < 0.05). The expression of BDNF mRNA was significantly increased in group E and M compared with the ones in group D(P<0.05). Conclusions ECT and propofol combined with ECT may up-regulate the expression of BDNF mRNA in hippocampus to improve depression symptoms of depressed rats. BDNF pathway wouldn' t participate the mechanism of ECT impair learning memory.