益心康泰胶囊对老年SD大鼠生精功能及血清超氧化物歧化酶和丙二醛的影响

目的:观察益心康泰对老龄SD大鼠精子密度和活率,以及血清超氧化物歧化酶(SOD)和丙二醛(MDA)的影响,并探讨其可能的作用机制。方法:将老年(24月龄)SD雄性大鼠随机分为空白组、十一酸睾酮组、益心康泰高、中、低剂量组共5组,根据体重,分别给予10 ml/kg生理盐水、4 mg/kg十一酸睾酮悬浊液、0.6 g/kg、0.3 g/kg、0.15 g/kg益心康泰悬浊液灌胃,1次/d,30 d后,取附睾,剥离脂肪、包膜,剪碎放入37℃恒温水浴锅中预热的生理盐水试管内,利用CASA精子自动分析系统(伟力)检测精子各项常规参数。取血查血清SOD和MDA含量。并行睾丸组织HE染色,在光镜下观察睾丸组织形态学变化。结果:与空白对照组相比,高、中、低剂量益心康泰均能显著提高老年大鼠的精子密度分别为(152.3±24.0)×106/ml、(334.5±97.7)×106/ml、(302.7±158.9)×106/ml、(221.4±75.6)×106/ml,P<0.05]和活率分别为(26.5±2.0)%、(35.5±5.9)%、(33.1±5.4)%、(28.4±2.1)%,高、中剂量与空白组相比P<0.01],同时,高剂量组可以显著提高血清SOD含量(22.0±5.3)U/ml,P<0.01],降低MDA含量(13.6±4.6)nmol/ml,P<0.01];睾丸组织学检测显示,给药组相对于空白组细胞增殖更为活跃,可见较多分裂期细胞,管腔内成熟精子数目较多,间质稀少,血管丰富,间质细胞发育良好。结论:益心康泰可以通过影响SOD和MDA的含量而改善老年SD大鼠的生精功能。

Effects of Yixin Kangtai Capsule on spermatogenesis and the levels of superoxide dismutase and malondialdehyde in aged SD rats

Objective: To observe the effects of Yixin Kangtai Capsule(YKC) on sperm concentration and motility,superoxide dismutase(SOD) and malondialdehyde(MDA) in aged SD rats,and to explore its possible mechanisms of action.Methods: We randomly assigned fifty 24-month-old male SD rats to five groups of equal number: blank control,testosterone undecanoate,high-dose YKC,medium-dose YKC and low-dose YKC,and treated them intragastrically with physiological saline at 10 ml/kg,testosterone undecanoate suspension at 4 mg/kg,and YKC at 0.6 g/kg,0.3 g/kg and 0.15 g/kg,respectively,once a day for 30 days.Then we harvested the epididymides,which were stripped of fat and envelope,cut into pieces and placed in the saline at 37 ℃ for 10 to 15 minutes.Then we detected the sperm parameters with the Weili CASA sperm analysis system,measured the levels of serum SOD and MDA,and,following HE staining,observed the histomorphological changes of the testicular tissue under the light microscope.Results:Compared with the controls,the rats treated with high-,medium-and low-dose YKC exhibited significantly increased sperm concentration(152.3±24.0]×106/ml vs 334.5±97.7],302.7±158.9] and 221.4±75.6]×106/ml,P<0.05) and markedly improved sperm motility(26.5±2.0]% vs 35.5±5.9],33.1±5.4] and 28.4±2.1]%,P<0.01).High-dose YKC significantly increased the level of serum SOD(22.0±5.3] U/ml,P<0.01) and reduced the content of MDA(13.6±4.6] nmol/ml,P<0.01).Testicular histology showed that YKC induced more active proliferation of cells,and increased the number of dividing cells and that of mature sperm in the lumen,with little interstitium,abundant blood vessels,and well-developed stromal cells.Conclusion: Yixin Kangtai Capsule can improve spermatogenesis in aged SD rats by regulating the levels of SOD and MDA.Natl J Androl,2012,18(9): 851-855