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| 大鼠血浆中甘草次酸浓度测定方法研究 | |
| 引用本文： | 王淑萍,曹波,张静泽,陈虹,高颖,白淑芳. 大鼠血浆中甘草次酸浓度测定方法研究[J]. 武警医学院学报, 2008, 17(3): 201-203 |
| 作者姓名： | 王淑萍 曹波 张静泽 陈虹 高颖 白淑芳 |
| 作者单位： | 武警医学院附属医院药剂科 天津300162(王淑萍)，武警医学院生药教研室 天津300162(曹波，张静泽，陈虹，高颖，白淑芳) |
| 摘 要： | [目的]建立一个血浆中甘草次酸浓度的测定方法。[方法]采用内标定量的反相高效液相色谱法,以甲醇-乙腈-水-醋酸（42：40：17：1）为流动相,色谱柱为C18（150 mm×4.6 mm,5μm）,检测波长为254 nm。[结果]在0.25-2.5μg/ml浓度范围内,线性关系良好（r=0.9989）。日内差RSD为0.88%。日间差RSD2.33%。甘草次酸的方法回收率为93.16%。[结论]本方法具有简便、灵敏、快速而准确的特点,适用于甘草次酸的血药浓度测定。 |
| 关 键 词： | 甘草次酸 高效液相色谱法 血药浓度 |
| 文章编号： | 1008-5041（2008）03-0201-03 |
| 收稿时间： | 2007-09-16 |
| 修稿时间： | 2007-11-18 |
Study on the method of determining plasma concentration of glycyrrhetinic acid |
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| WANG Shu-ping,CAO Bo,ZHANG Jing-ze,CHEN Hong,GAO Ying,BAI Shu-fang. Study on the method of determining plasma concentration of glycyrrhetinic acid[J]. Acta Academiae Medicinae CPAPF, 2008, 17(3): 201-203 | |
| Authors: | WANG Shu-ping CAO Bo ZHANG Jing-ze CHEN Hong GAO Ying BAI Shu-fang |
| Affiliation: | WANG Shu-ping, CAO Bo, ZHANG Jing-ze, CHEN Hong, GAO Ying, BAI Shu-fang(Department of Pharmacy, The Affiliated Hospital of Medical College of Chinese People＇s Armed Police Force, Tianjin 300162, China) |
| Abstract: | [Objective] To optimize the HPLC conditions for measuring plasma concentration of the glycyrrhetinic acid. [ Methods] The RP-HPLC with internal labeling was employed. Methanol-acetonitrile-H2 O-acetic acid（42： 40： 17： 1） was used as mobile phase and C18（150 mm×4.6 mm,5μm） column as fixed phase, glycyrrhetinicacid was detected by HPLC at length 254 nm. [Results] A linear plot of serum glycyrrhetinicacid versus peak area was within the range of 0.25- 2.5 μg/ml（ r = 0.9989） [Conclusions] The simple, rapid and sensitive HPLC conditions offers a method of plasma concentration of glycyrrhetinicacid. |
| Keywords: | Glycyrrhetinicacid HPLC Plasma concentration |
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