| HPLC法测定红杏止咳合剂中黄芩苷的含量 |
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| 引用本文： | 张瑗惠,臧恒昌. HPLC法测定红杏止咳合剂中黄芩苷的含量[J]. 齐鲁药事, 2012, 0(10): 579-580 |
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| 作者姓名： | 张瑗惠 臧恒昌 |
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| 作者单位： | [1]山东大学药学院,山东济南250012 [2]淄博市食品药品监督管理局张店分局,山东淄博255000 |
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| 摘 要： | 目的建立高效液相色谱法测定红杏止咳合剂中黄芩苷含量的方法。方法采用Shim-pack VP-ODS色谱柱(4.6 mm×250 mm,5μm),流动相为甲醇-1.5%冰醋酸(45∶55),检测波长274 nm,流速1.0 mL.min-1,柱温40℃。结果黄芩苷在0.061 0～2.440μg(r=1.000)范围内呈良好的线性关系;平均加样回收率为99.34%,RSD=1.5%(n=6)。结论该方法简便,准确,可用于红杏止咳合剂的质量控制。
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| 关 键 词： | 红杏止咳合剂 黄芩苷 高效液相色谱法 |
Determination of Baicalin in Hongxing Cough Mixture by HPLC |
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| ZHANG Yuan-hui,ZANG Heng-chang. Determination of Baicalin in Hongxing Cough Mixture by HPLC[J]. qilu pharmaceutical affairs, 2012, 0(10): 579-580 |
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| Authors: | ZHANG Yuan-hui ZANG Heng-chang |
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| Affiliation: | 1. School of Pharmaceutical Sciences, Shandong University, Jinan 250012, China ; 2. Zibo Zhangdian Food and Drug Administration, Zibo 255086, China) |
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| Abstract: | Objective To establish an HPLC method for the determination of baicalin in Hongxing Cough Mixture. Methods Shimpack VP- ODS column （4.6 mm × 250 mm,5 μm） was used;The mobile phase was methanol- 15% glacial acetic acid（47： 55） with the flow rate of 1.0 mL .min^-1 ; The detection wavelength was 274 nm and the column temperature was 40 ℃. Results The linear range of baicalin was 0. 061 0 - 2. 440 μg I r = 1. 000 ）. The average recovery was 99.34% , RSD = 1.5 % （ n = 6 ）. Conclusion The method was simple and accurate, and it can be used for the quality control of Hongxing Cough Mixture. |
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| Keywords: | Hongxing Cough Mixture Baicalin HPLC |
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