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| 介绍一种测定溶血的高灵敏度方法 | |
| 引用本文： | 胡望平,胡盈莹,冯福英,黄兢姚,张荣盛,陈荣仁,周长邵,王海林. 介绍一种测定溶血的高灵敏度方法[J]. 生物医学工程学杂志, 2007, 24(3): 664-666 |
| 作者姓名： | 胡望平 胡盈莹 冯福英 黄兢姚 张荣盛 陈荣仁 周长邵 王海林 |
| 作者单位： | 1. 南京军区福州总医院,生化科,福建医科大学福总临床医学院,福州,350025 2. 福州市传染病医院,肝病研究所,福州,350025 3. 福建医科大学,协和医院,福州,350025 |
| 摘 要： | 建立灵敏度高、试剂安全测定血浆游离血红蛋白(FHb)的方法。用于各项溶血试验。其方法为设计改良Trinder反应的2,4-二氯酚(2,4-DCP)方法,并进行各项性能验证。结果显示:本法灵敏度是酚法的2.39倍,批内批间重复性好。呈色稳定,FHb参考值为1～36.7mg/L,以液体枸橼酸钠抗凝剂,保存红细胞最优。FHb2,4-二氯酚法无毒、无癌原性,适用临床及生物医学工程学的各项溶血试验。 |
| 关 键 词： | 溶血试验 血浆血红蛋白 Trinder反应 体外循环 2,4-二氯酚法 |
| 修稿时间： | 2005-02-232005-11-21 |
Recommendation of a Highly Sensitive Method for Measuring Hemoglobin in Hemolytic Test |
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| Hu Wangping,Hu Yingying,Feng Fuying,Huang Jinyao,Zhang Rongsheng,Chen Rongren,Zhou Changshao,Wang Hailin. Recommendation of a Highly Sensitive Method for Measuring Hemoglobin in Hemolytic Test[J]. Journal of biomedical engineering, 2007, 24(3): 664-666 | |
| Authors: | Hu Wangping Hu Yingying Feng Fuying Huang Jinyao Zhang Rongsheng Chen Rongren Zhou Changshao Wang Hailin |
| Affiliation: | 1 .Clinical Chemistry Department,Fuzhou Genenal Hospital; Fuzhou 350025,China; 2 .Institute of Hepatology, Fuzhou Infectious Hospital, Fuzhou 350025,China; 3 .Union Hospital, Fujian Medical University,Fuzhou 350001,China |
| Abstract: | In this paper is recommended a highly sensitive and reagent-safe method to determine plasma heamoglobin(FHb)in viscacha hemolytic test.The 2,4-dichlorophenol method(2,4-DCP)of Trinder reaction has been improved.The performance of 2,4-DCP is verified.The sensitivity of 2,4-DCP is 2.39 times that of phenol method.It is well used with run precision and day-to-day precision.The reaction color is stable.The reference value FHb is 1-36.7 mg/L.Sodium citric is an excellent anticoagulant liquid to keep erythrocyte.The 2,4-DCP method is neither carcinogenic nor poisonous;it is suitable for viscacha hemolytic test in clinical and biomedical engineering. |
| Keywords: | Hemolytic test Plasma heamoglobin Trinder reaction Extracorporeal circulation 2 4-Dichlorophenol method |
| 本文献已被 CNKI 维普 万方数据 等数据库收录！ | |