|
|||||
|
|
| 角膜碱烧伤中PMNs与MMP-9的关系 | |
| 引用本文： | 宋东宇,高明宏,李冬梅.角膜碱烧伤中PMNs与MMP-9的关系[J].国际眼科杂志,2019,19(11):1844-1847. |
| 作者姓名： | 宋东宇 高明宏 李冬梅 |
| 作者单位： | 中国辽宁省朝阳市中心医院眼科; 中国北京市,首都医科大学附属北京同仁医院 北京同仁眼科中心 北京市眼科学与视觉科学重点实验室,中国辽宁省沈阳市,北部战区总医院眼科,中国北京市,首都医科大学附属北京同仁医院 北京同仁眼科中心 北京市眼科学与视觉科学重点实验室 |
| 基金项目： | 首都卫生发展科研专项项目(No.2018-2-2053) |
| 摘 要： | 目的：研究角膜碱烧伤后基质损伤修复病程中多形核中性白细胞(PMNs)的浸润和基质金属蛋白酶-9(MMP-9)的表达的关系。 方法：建立25只家兔角膜碱烧伤模型，分别于伤后3、7、14、21、28d随机处死5只兔，裂隙灯下观察角膜病理修复情况，摘除角膜做病理切片，测定PMNs的浸润量值。免疫组化法测定MMP-9的表达。 结果：PMNs和MMP-9在角膜碱烧伤后的3d开始升高，14d上升达到最大峰值，之后逐渐降低，碱烧伤后角膜基质在第14d溃疡面积及深度最为严重。 结论：角膜碱烧伤病灶中PMNs和MMP-9的量值呈正相关，且角膜的病理损伤与PMNs的浸润和MMP-9的表达密切相关。 |
| 关 键 词： | 角膜碱烧伤 基质金属蛋白酶-9 多形核中性白细胞 |
| 收稿时间： | 2019/5/9 0:00:00 |
| 修稿时间： | 2019/9/27 0:00:00 |
Effect of PMNs and MMP-9 on corneal injury after alkali burn |
|
| Dong-Yu Song,Ming-Hong Gao and Dong-Mei Li.Effect of PMNs and MMP-9 on corneal injury after alkali burn[J].International Journal of Ophthalmology,2019,19(11):1844-1847. | |
| Authors: | Dong-Yu Song Ming-Hong Gao and Dong-Mei Li |
| Institution: | Department of Ophthalmology, Chaoyang Central Hospital, Chaoyang 122000, Liaoning Province, China; Beijing Tongren Eye Center, Beijing Tongren Hospital, Capital Medical University, Beijing Ophthalmology & Visual Sciences Key Lab, Beijing 100730, China,Department of Ophthalmology, General Hospital of Northern Theater Command, Shenyang 110016, Liaoning Province, China and Beijing Tongren Eye Center, Beijing Tongren Hospital, Capital Medical University, Beijing Ophthalmology & Visual Sciences Key Lab, Beijing 100730, China |
| Abstract: | AMI: To study the relationship between the infiltration of polymorphonuclear neutrophils(PMNs)and the expression of matrix metalloproteinases 9(MMP-9)in corneal stroma injury after alkali burn. METHODS: Cornea alkali-burned model was made in 25 rabbits, then animals were grouped and sacrificed at 3d, 7d, 14d, 21d and 28d, respectively. The condition developing of alkali-burned cornea was observed by slit lamp biommicroscopy. The corneas were enucleated for histopathological examination. The infiltration of PMNs was identified by hematoxylin eosin(HE)staining and the expression of MMP-9 was identified by immunohistochemisty in different periods. RESULTS: The quantity of PMNs and MMP-9 increased on the 3d, reached the lower level on 7d, shown a peak on the 14d, then decreased gradually. The area and depth of corneal stroma after alkali burn were the most severe on the 14d. CONCLUSION: During the wound healing process, alkali-burned cornea has close relation with the infiltration of PMNs and the expression of MMP-9. The infiltration of PMNs and the expression of MMP-9 is positively correlated in corneal stroma injury after alkali burn. |
| Keywords: | alkali-burned cornea matrix metalloproteinase-9 polymorphonuclear neutrophils |
| 点击此处可从《国际眼科杂志》浏览原始摘要信息 | |
| 点击此处可从《国际眼科杂志》下载免费的PDF全文 | |