| 女贞子多糖相对分子质量与组成分析 |
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| 引用本文： | 战兴晓,刘希光,刘祥龙,吕静,孙敬林,孙祥斌. 女贞子多糖相对分子质量与组成分析[J]. 中草药, 2019, 50(18): 4288-4292 |
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| 作者姓名： | 战兴晓 刘希光 刘祥龙 吕静 孙敬林 孙祥斌 |
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| 作者单位： | 鲁东大学化学与材料科学学院, 山东 烟台 264000,鲁东大学化学与材料科学学院, 山东 烟台 264000,鲁东大学化学与材料科学学院, 山东 烟台 264000,鲁东大学化学与材料科学学院, 山东 烟台 264000,鲁东大学化学与材料科学学院, 山东 烟台 264000,鲁东大学化学与材料科学学院, 山东 烟台 264000 |
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| 摘 要： | 目的透析进一步纯化女贞子多糖,通过对女贞子多糖相对分子质量及组成分析,为探究多糖生物活性与其组成之间的关系提供理论数据。方法实验以女贞子为研究对象,采用水提醇沉法提取女贞子多糖,通过Sevage法、过氧化氢、透析等方法纯化多糖,凝胶渗透色谱分析多糖相对分子质量,三氟乙酸水解后采用高效液相色谱-示差检测器分析多糖组成。结果女贞子多糖重均相对分子质量为10721,数均相对分子质量为10673,女贞子多糖由葡萄糖、鼠李糖、阿拉伯糖3种单糖所组成,其物质的量比为9.14∶8.10∶5.18。结论此种方法纯化后的女贞子多糖组成较为均一,纯度较高,采用高效液相色谱-示差检测器无需柱前衍生化即可分析多糖中单糖组成。
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| 关 键 词： | 女贞子多糖 透析 相对分子质量 高效液相色谱-示差检测器 单糖组成 |
| 收稿时间： | 2019-02-03 |
Molecular weight and composition determination of polysaccharide from Ligustrum Lucidum |
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| ZHAN Xing-xiao,LIU Xi-guang,LIU Xiang-long,LV Jing,SUN Jing-lin and SUN Xiang-bin. Molecular weight and composition determination of polysaccharide from Ligustrum Lucidum[J]. Chinese Traditional and Herbal Drugs, 2019, 50(18): 4288-4292 |
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| Authors: | ZHAN Xing-xiao LIU Xi-guang LIU Xiang-long LV Jing SUN Jing-lin SUN Xiang-bin |
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| Affiliation: | School of Chemistry and Materials Science, Ludong University, Yantai 264000, China,School of Chemistry and Materials Science, Ludong University, Yantai 264000, China,School of Chemistry and Materials Science, Ludong University, Yantai 264000, China,School of Chemistry and Materials Science, Ludong University, Yantai 264000, China,School of Chemistry and Materials Science, Ludong University, Yantai 264000, China and School of Chemistry and Materials Science, Ludong University, Yantai 264000, China |
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| Abstract: | Objective To study the relative molecular mass and composition of Ligustrum lucidum polysaccharide purified by dialysis, and provide the theories for the relationship between the biological activity and the internal composition. Methods In this paper, L. lucidum as an object was studied. Polysaccharide was isolated by water extraction and ethyl alcohol precipitation and purified by Sevage method, hydrogen peroxide and dialysis. After the acidolysis of trifluoroacetic acid, the molecular weight was measured by GPC, and the composition of polysaccharide was analyzed by HPLC-RID. Results The Mw of polysaccharide was 10 721, and the Mn of polysaccharide was 10 673. L. lucidum polysaccharide consisted of glucose, rhamnose, and arabinose, the molar ratio of these monosaccharide was 9.14:8.10:5.18. Conclusion The purified polysaccharide composition is more homogeneous, and the monosaccharides of polysaccharides was easily analyzed by HPLC-RID without column derivatization. |
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| Keywords: | Ligustrum lucidum polysaccharide dialysis relative molecular mass HPLC-RID monosaccharide composition |
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