谷氨酰胺对脓毒症大鼠急性肠道功能障碍的保护作用

目的 探讨谷氨酰胺对脓毒症大鼠急性肠道功能障的保护作用。方法S 将SD大鼠随机分为3组,即正常组(A组),模型组(B组)及谷氨酰胺组(C组), 模型组及谷氨酰胺组大鼠腹腔注射0.45mg/ml的LPS溶液,1ml/100g,即4.5mg/Kg,10分钟内分3次注射完毕进行造模。模型组造模后12小时给予5次/天灌注百普素溶液灌胃；谷氨酰胺组造模后12小时给予5次/天灌注百普素溶液,浓度为25.2％(80 kcal),每次4ml,另灌胃谷氨酰胺3.75g/Kg/d。模型组随机分为喂养36小时组(B1组)和喂养72小时组(B2组)。对各组进行组织病理观察,同时检测各组肠组织谷氨酰胺浓度。结果1. 模型36小时组、模型72小时组肠组织谷氨酰胺浓度低于正常组(P<0.05)；模型36小时组、模型72小时组、谷氨酰胺组肠黏膜Chiu氏评分、绒毛长度及黏膜层厚度与正常组比较差异有统计学意义(P<0.05)；模型组肠黏膜组织超微结构变化较谷氨酰胺组明显。2. 谷氨酰胺组肠组织谷氨酰胺浓度较模型36小时组、模型72小时组升高(P<0.05)；谷氨酰胺组肠组织超微结构变化较模型组不同程度减轻。结论S脓毒症大鼠肠组织谷氨酰胺浓度下降,存在肠功能障碍,给予谷氨酰胺后急性肠道功能障得到保护。

Effect of glutamine on Septic Acute Intestinal Dysfunction in Rats

Objective: To investigate the effect of glutamine on septic acute intestinal dysfunction (SAID) in a rat model. Methods: SD rats were divided into three groups: a normal group (A), a model group (B), and a glutamine group (C). Group B was divided into a 36-h group (B1) and a 72-h feeding group (B2). The concentrations of intestinal glutamine and histopathological changes were measured. Results: Intestinal glutamine levels of groups B1 and B2 (1.023±0.039 and 1.651±0.036, respectively) were lower than groups A (2.924±0.135, all P<0.05).The lengths of the villus and thicknesses of the mucosal layer in groups B1, B2 and C were significantly different from those in group A. The ultrastructural changes of intestines in group B were obvious, while those of group C were not. Intestinal glutamine levels of groups B1 and B2 (1.023±0.039 and 1.651±0.036, respectively) were lower than groups C (2.320±0.157, all P<0.05).The damage of mucosal were lessened of groups C. Conclusions: Treatment with glutamine can decrease intestinal mucosal injury of SAID.