杜仲皮化学成分研究

目的研究杜仲Eucommia ulmoides皮的化学成分。方法采用正相硅胶柱色谱、反向ODS柱色谱、Sephadex LH-20柱色谱、制备HPLC等手段对杜仲皮化学成分进行分离纯化,并根据HR-ESI-MS、NMR等波谱数据进行结构鉴定。结果从杜仲皮95%乙醇提取物中分离得到9个化合物,鉴定为甘草黄酮B(1)、3,5,4′-三羟基-7,3′-二甲氧基黄酮(2)、甘草素(3)、formononetine(4)、(+)-4′-O-methylglabridin(5)、paratocarpin E(6)、龙血素C(7)、threo-1-(4-hydroxy-3-methoxyphenyl)-1-methoxy-2-{4-1-formyl-(E)-vinyl]-2-methoxyphenoxy}-3-propanol(8)、diospyrosin(9),其中7个黄酮和2个苯丙素类。结论化合物1～9均首次从杜仲属植物中分离得到。

Chemical constituents from barks of Eucommia ulmoides

Objective To study the chemical constituents of barks of Eucommia ulmoides. Methods Compounds were isolated by means of normal phase silical gel, reverse phase ODS, Sephadex LH-20 column chromatography, and preparative-HPLC, and their structures were identified on the basis of various spectroscopic analyses, including NMR and HR-ESI-MS. Results Nine known compounds, including seven flavonoids and two phenylpropanoids, were isolated from the 95% EtOH extract of barks of E. ulmoides. Their structures were identified as licoflavone B (1), 3,5,4''-trihydroxy-7,3''-dimethoxyflavone (2), liquiritigenin (3), formononetine (4), (+)-4''-O-methylglabridin (5), paratocarpin E (6), loureirin C (7), threo-1-(4-hydroxy-3-methoxyphenyl)-1-methoxy-2-{4-1-formyl-(E)-vinyl]-2-methoxyphenoxy}-3-propanol (8), and diospyrosin (9). Conclusion Compounds 1-9 are obtained from the genus of Eucommia for the first time.