PTEN和p53在视网膜母细胞瘤中的表达及临床意义

目的检测视网膜母细胞瘤(retinoblastoma,RB)组织中PTEN基因和p53基因的蛋白表达水平并探讨二者在RB发生发展中的作用以及相关性。方法采用PV-9000免疫组织化学二步法,检测40例RB组织和16例正常视网膜组织中PTEN和p53蛋白的表达,统计分析不同临床和病理阶段RB表达PTEN和p53的差异及其相关性。结果(1)PTEN在正常视网膜组织中的阳性表达率为100%,显著高于RB55%(P<0.01);PTEN蛋白的阳性表达率与临床分期、病理分型以及是否伴有视神经浸润有关(P<0·05);与性别差异无关(P>0.05)。(2)p53在正常视网膜组织中不表达,在RB组织中阳性表达率为57.5%,差异有统计学意义(P<0·05);p53蛋白的阳性表达率与临床分期、病理分型以及是否伴有视神经浸润有关(P<0.05);与性别差异无关(P>0·05)。(3)PTEN和p53在RB中的蛋白表达呈负相关(r=-0.3710,P<0.05)。结论PTEN和p53可作为判断RB恶性程度和预后的指标。PTEN可作为RB早期诊断的分子标记物之一。

Expression and clinical significance of PTEN and p53 in retinoblastoma

Objective To investigate the protein expression of PTEN and p53 in retinoblastoma (RB),to approach the correlation of two genes in the development of retinoblastoma.Methods Forty retinoblastoma samples,sixteen normal retinal tissues were collected.The expression of PTEN and p53 protein was evaluated by immunohistochemistry PV-9000 method.The results associated with clinical pathological features were analyzed.Results In normal retina,the positive rates of PTEN protein expression were 100%,which were significantly higher than that in retinoblastoma (55%) (P<0.01).There were no relationship between the positive rates of PTEN protein expression and sex differences(P>0.05),but there was significant difference between the positive rates of PTEN protein expression and clinical stage,pathological typing and whether or not accompanied optic nerve infiltration by statistics(P<0.05).In retinoblastoma,the positive rate of p53 protein expression was 57.5%,which was significantly higher than that in normal retina(0%)(P<0.05).There were no relationship between the positive rates of p53 protein expression and sex differences(P>0.05),but there were significant difference between the positive rates of p53 protein expression and clinical stage,pathological typing and whether or not accompanied optic nerve infiltration by statistics(P<0.05).There was a negative correlation between PTEN and p53 in retinoblastoma(r=-0.3710,P<0.05).Conclusion PTEN gene may be a molecule tumor marker for early diagnosis of retinoblastoma.PTEN gene and p53 may be the indexes to evaluate malignant degree and prognosis of RB.