72000IV型胶原酶的分泌和活化与肿瘤细胞转移潜能关系的研究

目的探讨肿瘤细胞转移潜能与７２０００ＩＶ型胶原酶的关系。方法采用明胶酶谱分析法,检测两组５种不同转移潜能的人癌细胞系,分泌７２０００ＩＶ型胶原酶的情况,以及人正常纤维母细胞在不同癌细胞的作用下,分泌的７２０００ＩＶ型胶原酶活性的改变。结果高转移潜能的癌细胞（ＰＧ、ＷＭ４５１和ＷＭ９８３ａ）分泌７２０００ＩＶ型胶原酶的量明显高于低转移潜能的癌细胞（ＰＡａ和ＷＭ３５）;在受ＰＧ和ＷＭ４５１细胞作用的纤维母细胞的条件培养液中,活化型７２０００ＩＶ型胶原酶的产量明显高于其他癌细胞作用的纤维母细胞。结论７２０００ＩＶ型胶原酶的表达和分泌与肿瘤细胞的转移潜能密切相关;高转移潜能的癌细胞（ＰＧ）和转移瘤细胞（ＷＭ４５１）可能通过分泌某些可溶性介质,刺激纤维母细胞,促进酶原型７２０００ＩＶ型胶原酶的活化,从而更有效地降解ＩＶ型胶原,达到转移的目的。

A study on the correlation between the activity of 72,000 type IV collagenase and the metastatic potential of cancer cells

Objective To evaluate the enzyme activity of 72000 type IV collagenase and its relationship with the metastatic potential of cancer cells. Methods The levels of secreted 72000 type IV collagenase in the conditioned media of five human cancer cell lines with different metastatic potential and a normal lung fibroblast strain treated with cancer cell culture media were examined by gelatin zymography and densitometric analysis. Results The levels of 72000 type IV collagenase secreted by cancer cells with high metastatic potential (PG, WM451 and WM983a) were higher than those secreted by cancer cells with low metastatic potential (PAa and WM35). In the conditioned media of fibroblasts which were treated with the culture media of PG and WM451, enhanced levels of activation were observed. Conclusion The secretion of 72000 type IV collagenase is closely correlated to the metastatic potential of cancer cells. The cancer cells with high metastatic potential may possibly through certain soluble mediators stimulate normal fibroblasts to activate 72000 type IV procollagenase.