APP/PS1转基因小鼠大脑内锌转运蛋白3的表达

目的研究锌转运蛋白3(ZnT-3)在APP/PS1转基因小鼠大脑皮质及海马内的分布和表达变化。方法应用免疫组织化学技术检测ZnT-3在APP/PS1转基因小鼠脑内的分布,并应用Western blot方法分析ZnT-3在大脑皮质及海马的表达水平。结果ZnT-3主要分布于APP/PS1转基因小鼠脑内的老年斑中,且主要定位于老年斑周围变性的神经元及其突起内;ZnT-3在APP/PS1转基因小鼠大脑皮质和海马的表达均明显高于野生型小鼠。结论ZnT-3在APP/PS1转基因小鼠大脑内的高表达以及在老年斑内的定位,提示ZnT-3可能在老年斑内锌离子的聚集过程中起着重要的调节作用,进而参与了APP/PS1转基因小鼠大脑内Aβ老年斑的形成。

Expression of Zinc Transporter-3 in APP/PS1 Transgenic Mouse Brain

Objective To investigate the expression change and distribution of zinc transporter-3(ZnT-3)in the hippocampus and cortex of APP/PS1 transgenic mouse.Methods The distribution of ZnT-3 in the APP/PS1 transgenic mouse brain was analyzed with immunohistochemistry,and Western blot technique was used to analyze the expression level of ZnT-3 in the hippocampus and cortex of APP/PS1 transgenic mouse.Results ZnT-3 mainly distributed in the senile plaques in the APP/PS1 transgenic mouse brain.ZnT-3 immunoreactivity was strong in the degenerating neurites in the peripheral of the plaque.Western blot results showed high expression of ZnT-3 in the cortex and hippocampus of APP/PS1 transgenic mouse compare with wild-type mouse.Conclusion The overexpression of ZnT-3 and localization of ZnT-3 in the plaques of the APP/PS1 transgenic mouse brain suggest the important role of ZnT-3 in regulating zinc accumulation during the pathological process of AD.