S100B和EGFR在特异性肺癌脑转移细胞中的表达和意义

目的：分析S100B和EGFR在特异性肺癌脑转移细胞株中的表达，探讨肺癌脑转移的机制。方法：免疫组化和western blotting检测特异性肺癌脑转移细胞株PC14／B中S100B、EGFR的表达情况，并与原株PCI4、非脑转移肺癌细胞株A549比较。结果：PC14／B中特异性表达2种由S100B参与形成的蛋白聚合体，且这两种蛋白在A549和PCI4中未见表达。EGFR在A549中的表达水平明显高于在PC14和PC14／B中的表达水平。结论：一些含有S100B的蛋白聚合体可能促进了肺癌脑转移的发生，EGFR可能同肺癌的脑转移行为呈负相关。

Expressions and significances of SIOOB and EGFR in highly brain metastatic subclone of a human lung cancer cell line

Objective：To investigate some surface cytokines expressed in a highly brain metastatic subclone of a human lung cancer cell line aiming to study the mechanism of brain metastasis in lung cancer. Methods： The expre -ssion levels of S100B and EGFR in PC14/B were detected by immunohistochemistry and Western Blotting analysis and compared with PC14 and A549. Results： We have detected two polymerides specificly expressed in PC14/B which S100B forms, they were not seen in both PC14 and A549. EGFR were expressed in A549 at a significantly higher level than in PCI4 and PC14/B. Conclusion： Some polymerides with S100B forms may promote the metastasis of lung cancer, and EGFR may have negative correlation with brain metastasis of lung cancer.