大鼠正畸牙齿移动过程中Wnt3a和DKK1的表达

目的:观察Wnt3a和DKK1在大鼠正畸牙移动时牙周组织中的表达变化。方法:将30只健康雄性SD大鼠随机分为6组,即正畸加力1,3,5,7,10,14 d组。使用镍钛拉簧施加50 g力近中移动左侧上颌第一磨牙,以右侧上颌第一磨牙不加力为自身对照。通过免疫组化方法检测牙周组织中Wnt3a和DKK1蛋白的表达。结果:Wnt3a和DKK1在加力组和未加力组的牙周膜中都有表达。在张力区,Wnt3a和DKK1都先增加后减少,分别在第5天和第10天达到高峰;在压力区,Wnt3a先减少后增加,DKK1先增加后减少,分别在第5天和第10天达峰值。结论:Wnt3a和DKK1参与牙周组织的改建,提示Wnt信号通路可能是正畸牙周改建的调控途径之一。[关键词] Wnt3a DKK1 正畸牙齿移动 牙周组织改建

Expression of Wnt3a and DKK1 during Orthodontic Tooth Movement.

Objective: To observe the changes of Wnt3a and DKK1 expressions in periodontal tissue during orthodontic tooth movement (OTM). Methods: Thirty male SD rats were randomly divided into six groups according to tooth movement time points of 1d, 3d, 5d, 7d, 10d, 14d. Nickel-titanium closed-coil springs were used to deliver an initial 50 g mesial force to the left maxillary first molars. The right maxillary molars without force application served as control. Immunohistochemical staining technology was used to detect the expression of Wnt3a and DKK1. Results: Wnt3a and DKK1were expressed on both the ipsilateral and contralateral sides in each group. On the tension side, expression of Wnt3a and DKK1 elevated firstly and then decreased, peaked at day 5 and day 7 respectively. On the compression side, Wnt3a expression decreased fisrtly and then increased while DKK1 quite the opposite, peaked at day 5 and day 10 respectively. Conclusion: Wnt3a and DKK1 participated in periodontal tissue remodeling, suggesting that Wnt signaling pathway may be one of regulation pathways in periodontal tissue remodeling during OTM.