miR-21通过下调PTEN表达促进肝癌细胞增殖、侵袭及迁移

目的 探讨miR-21在肝癌组织中的表达、临床意义及可能的分子机制。方法 实时定量PCR检测miR-21在肝癌及癌旁组织中的表达，MTT法检测MHCC-97H细胞的增殖，Transwell实验检测MHCC-97H细胞的侵袭及迁移，Westernblot法检测miR-21下游潜在靶点PTEN的表达。结果 miR-21在肝癌组织中表达水平显著高于相应癌旁组织;肝癌组织中miR-21高表达与肿瘤大小、TNM分期、门静脉侵犯及Edmondson分级显著相关;高表达miR-21患者3年生存率明显低于低表达组;miR-21可显著促进MHCC-97H细胞的增殖、侵袭及迁移，并下调PTEN的蛋白水平。结论 miR-21在肝癌组织中表达显著上调并与肝癌恶性临床病理特征有关，miR-21促进肝癌细胞增殖、侵袭及迁移的作用可能与下调PTEN表达有关。

miR-21 promotes the proliferation,invasion and migration ofhepatocellular carcinoma cell via down-regulation of PTEN expression

Objective To investigate the level and clinical significance of miR-21 expression in human hepatocellular carcinoma(HCC)and the underlying mechanisms.Methods Real time quantitative PCR was applied to detect the expression of miR-21 in HCC and the corresponding tumor-adjacent tissues.The proliferation of MHCC-97H cells were detected by MTT assay.Transwell assay was performed to measure the invasion and migration of MHCC-97H cells.The expression of phosphatase and tensin homolog (PTEN) was determined by Western blotting.Results The expression level of miR-21 in HCC tissues was significantly higher than that in the adjacent normal tissues,and it was significantly associated with tumor size,TNM stage,portal vein infiltration and Edmondson degree.Patients in the higher miR-21 group had a worse 3-year survival than those in lower miR-21 group.Down regulation of miR-21 in MHCC-97H cells significantly suppressed cell proliferation,invasion and migration,and increased the protein expression of PTEN.Conclusion The expression of miR-21 in HCC tissue is significantly higher than that in tumor-adjacent normal tissues and was correlated with the malignant clinical pathological features.miR-21 promotes HCC cell proliferation,migration and invasion by inhibiting the expression of PTEN.