IL-17在高危型HPV阳性宫颈癌中的表达

目的： 通过检测高危型人乳头瘤病毒（HPV）阳性宫颈癌患者体内IL-17的表达水平，探讨IL-17所介导的特异性免疫反应在高危型HPV感染的宫颈癌发生、发展中可能发挥的作用。 方法： 选取高危型HPV阳性的宫颈浸润性鳞癌患者（宫颈癌组）28例、宫颈高危型HPV感染者（HPV感染组）25例以及对照组30例。采用ELISA法检测各组患者外周血清及宫颈局部组织匀浆上清液中IL-17 的表达水平。 结果： ①宫颈癌组患者外周血清中IL-17水平较对照组明显升高（ P＜0.05），与HPV感染组比差异无统计学意义（均P＞0.05），HPV感染组与对照组间差异无统计学意义（ P＞0.05）；②各组宫颈局部组织匀浆上清液中IL-17的表达水平均明显高于外周血清表达水平，差异均有统计学意义（均P＜0.05）；③宫颈局部组织匀浆上清液中IL-17表达水平各组间的差异均有统计学意义（均P＜0.05），且宫颈癌组局部组织匀浆上清液中IL-17的表达水平高于HPV感染组，HPV感染组高于对照组，差异均有统计学意义（均P＜0.05）。 结论：IL-17所介导的免疫反应可能参与并促进了高危型HPV的持续性感染，与宫颈癌的发生、发展进程相关。

The expression of IL-17 in the cervical cancer with high-risk human papillomavirus infection#br#

Objective: To investigate the expression of IL-17 in patients diagnosed as cervical squamous cell carcinoma (SCC) with high-risk human papillomavirus (HPV) infection, with the aim to explore the potential role of specific immune response mediated by IL-17 in the disease progression and carcinogenesis of cervical cancer which is closely associated with high-risk HPV infection. Methods: The study groups included 28 cervical SCC patients with high-risk HPV infection and 25 high-risk HPV infected women with normal cervical cytology. Thirty patients diagnosed as uterine myoma with normal cervical cytology and negative HPV testing were enrolled as the control group. The concentration of IL-17 in serum and supernatant of cervical tissue homogenate were examined with ELISA method. Results: ①The serum concentrations of IL-17 in patients with cervical SCC were significantly higher than those in the control group (P<0.05), but there was no significant difference observed between SCC group vs the high-risk HPV infection group (P>0.05) and the high-risk HPV infectiongroup vs the control group (P>0.05); ②The average concentrations of IL-17 in supernatant of cervical tissue homogenate of all groups were obviously higher than those in serum respectively (P<0.05); ③The concentrations of IL-17 in supernatant of cervical tissue homogenate differed significantly among the study groups and the control group (P<0.05). The concentrations in the cervical SCC group were higher than those in the high-risk HPV infected group, which were much higher than the control group (P<0.05). Conclusion: These results indicate that the immune reaction mediated by IL-17 may participate in and promote the persistent infection of high-risk HPV,which is closely associated with the disease progression and carcinogenesis of cervical cancer cervical cancer.