Leptin、NF-κBp65在子宫颈上皮内病变组织中的表达及意义

目的 探讨瘦素（leptin）、核转录因子-κBp65（nuclear factor κBp65，NF-κBp65）与子宫颈上皮内瘤变（cervical inraepithelial neoplasia，CIN）发生、发展的关系。方法 采用免疫组化染色MaxVisionTM法检测63例慢性子宫颈炎（炎症组）、125例CIN组织（CIN组）中Leptin 和NF-κBp65的表达。结果 Leptin在炎症组中100%表达，且呈强阳性表达，在CIN组中随着CIN级别的升高其表达逐渐减弱；NF-κBp65在炎症组和CIN组织中阳性表达率均为100%，CIN组为强阳性表达，其表达强度随CIN 级别升高而增强，差异有统计学意义（P＜0.05）。Leptin和NF-κBp65的表达强度呈负相关（r =-0.234 ，P＜0.05）。结论 NF-κBp65的高表达对CIN的发生、发展起促进作用。在CIN发生、发展过程中，可能由于细胞异常增生影响Leptin合成与分泌而致其表达下降或异常。

Expression and clinical significance of Leptin and NF-κBp65 in cervical intraepithelial neoplasia

Objective To explore the expression of Leptin and NF-κBp65 in cervical intraepithelial neoplasia and its clinical significance. Methods Expression of Leptin and NF-κBp65 was analyzed immunohistochemically in 63 patients with chronic cervicitis and 125 patients with cervical intraepithelial neoplasia. Results Leptin expression was detected in 100% of samples from patients with chronic cervicitis，and staining intensity was strong. NF-κBp65 was detected in 100% of samples from both groups of patients. NF-Bp65 staining intensity was significantly higher in cervical intraepithelial neoplasia than in chronic cervicitis，and intensity increased with severity of neoplasia. Leptin and NF-κBp65 expression correlated negatively in both sets of patients. Conclusion High expression of NF-κBp65 promotes the development of cervical intraepithelial neoplasia. As this disease develops， Leptin expression decreases and becomes abnormal.