RGD多肽修饰的无机小牛骨粉对MC3T3-E1细胞黏附、增殖及分化的影响

目的:研究小鼠前成骨细胞MC3T3-E1在RGD修饰的无机小牛骨粉上的黏附、增殖及分化。方法:浸泡法制备RGD/Bio-Oss复合材料。MTT法检测细胞1 h、2 h、3 h黏附,扫描电镜观察细胞黏附形态,DNA浓度定量分析细胞1 d、3 d、5 d、7 d的增殖。碱性磷酸酶(ALP)试剂盒检测细胞7 d、14 d的分化。结果:1 h、2 h、3 h细胞黏附结果Bio-Oss/RGD组明显高于Bio-Oss组及空白对照组;1 d、3 d、5 d、7 d Bio-Oss/RGD组和Bio-Oss组的DNA浓度差别无统计学意义,但都低于空白对照组;细胞分化第14天时Bio-Oss/RGD组的结果明显高于Bio-Oss组与空白对照组。结论:RGD对细胞黏附及分化有一定的促进作用,但对细胞增殖无显著促进作用。

Adhesion,Proliferation and Differentiation of MC3T3-E1 Cell on Bovine Bone Substitute Modified with RGD Peptide.

Objective: To evaluate the effect of adhesion, proliferation and differentiation of MC3T3-E1 cells on the bovine bone modified by RGD peptide. Methods: In RGD/Bio-Oss group, Bio-Oss was coated with RGD using only deep and dry methods. The MC3T3-E1 cells were seeded on the RGD modified Bio-Oss, after 1h, 2h and 3h, the adhesion of MC3T3-E1 cells was determined by MTT assay. The morphology of MC3T3-E1 cells was observed by SEM. The proliferation of MC3T3-E1 cells was determined by DNA concentration after 1d, 3d, 5d and 7d. The MC3T-E1 cells were cultured on RGD modified Bio-Oss for 7d and 14d with osteogenic supplements, the ALP activity of the MC3T3-E1 cells was measured. Results: The cell adhesion of the RGD modified Bio-Oss group was much higher than the unmodified Bio-Oss group and control group. No significant difference in cell DNA concentration between modified and unmodified Bio-Oss after 1d, 3d, 5d and 7d culture, but lower than control group. On the 14d osteogenesis inducing, the ALP activity of the cultured MC3T3-E1 cells on modified Bio-Oss was higher than unmodified Bio-Oss group and control group. Conclusion: RGD peptide could enhance MC3T3-E1 cells adhesion on Bio-Oss and promote osteogenesis differentiation, but no obvious proliferation effects.