法舒地尔对阿霉素致大鼠心肌损伤的保护作用

目的 探讨法舒地尔（fasudil，Fas）干预对阿霉素（adriamycin，ADM）所致大鼠心肌损伤的保护作用，为ADM化疗的心肌保护用药提供实验依据。方法 将32只SD大鼠随机分为4组：A组（空白对照组），B组（ADM组），C组［ADM+Fas 2.5 mg/（kg·d）组］，D组［ADM+Fas　10　mg/（kg·d）组］，每组8　只。分别于T1（实验开始前）、T2（实验第3天）、T3（实验第15天）、T4（实验第20天）四个时点采集大鼠血液标本，用ELASA法检测大鼠血清氨基末端B型利钠肽原（NT-pro BNP）、心型脂肪酸结合蛋白（H-FABP）、心肌肌钙蛋白I（cTnI）的水平（简称三联指标）；观察各组大鼠心肌组织病理学变化并进行评分。结果 ⑴大鼠血清NT-pro BNP、H-FABP、cTnI的水平比较： A组大鼠中三联指标在T1、T2、T3、T4四个时间点的水平比较无明显差异（P>0.05）；B组大鼠中， T2、T3、T4时间点的三联指标均明显高于T1，差异均有统计学意义（P均<0.05）；C组、Ｄ组大鼠中， T2、T3、T4时间点的三联指标均明显高于T1（P均＜0.05），而T4比T2时间点明显降低，二者差异有统计学意义（P<0.05）。⑵大鼠心肌组织病理学评分：与A组相比，B组、C组、D组大鼠心肌组织病理学评分均明显增高（P＜0.05）；与B组相比，C组、D组大鼠心肌组织病理学评分均明显降低（P<0.05），其中D组明显低于C组，差异有统计学意义（P<0.05）。 结论 法舒地尔干预对阿霉素所致大鼠的心肌损伤具有一定的保护作用。

Protective effects of Fasudil in adriamycin-induced myocardial injury in rats

Objective The aim of this study was to explore the potential myocardial protective effects of Fasudil in a rat model of adriamycin-induced myocardial injury. Methods Sprague-Dawley rats（n=32） were randomly divided into groups that received no treatment， adriamycin， adriamycin+Fasudil （2.5 mg per kg per day），or adriamycin+Fasudil （10 mg per kg per day），8 rats in every group. Blood samples were collected from animals at baseline and on days 3，15 and 20 after treatment began. Serum levels of NT-pro BNP，H-FABP and cTnI were measured by ELISA，and the degree of myocardial injury was determined based on histopathology. Results In animals treated with adriamycin， levels of NT-pro BNP， H-FABP and cTnI were significantly higher at the three time points after treatment than at baselin（P<0.05）. In animals treated with adriamycin and either dose of Fasudil， levels of the three serum markers were also significantly higher at the three time points after treatment than at baseline；however， the levels decreased during follow-up，such that they were significantly lower on day 20 than on day 3（P<0.05）.  Myocardial pathology scores were significantly higher in all treatment groups than in the control group， but scores in animals treated with adriamycin together with either dose of Fasudil were significantly lower than scores in animals treated with adriamycin alone （P<0.05）. Between the two groups treated with Fasudil， pathology score was significantly lower with the higher dose than with the lower one （P<0.05）. Conclusion Fasudil may help protect against adriamycin-induced myocardial injury in a rat model.