人胃癌细胞系MKN-45中二甲双胍对顺铂的拮抗作用

 目的 初步研究二甲双胍(metformin,Met)和Met联合顺铂(cisplatin, Cisp)对人胃癌MKN-45细胞系的抗癌作用。方法 应用不同浓度Met和Cisp单独或联合处理人胃癌MKN-45细胞系,MTT法分析细胞存活率,RT-PCR检测生存素、mTOR和Akt的mRNA表达水平,流式细胞仪检测细胞凋亡。结果 单独使用Met可抑制增殖并诱导凋亡;Met联合Cisp时,可降低Cisp的抗增殖作用,且生存素、Akt和mTOR的mRNA表达明显增加(P＜0.05)。Met对Cisp的拮抗作用可能是通过生存素、Akt和mTOR信号通路调节。结论 Met能抑制人胃癌MKN-45细胞系对Cisp化疗的敏感性,在患有2型糖尿病的胃癌患者中,应该慎重将Met与Cisp联合使用。

Preliminary study on the antagonistic effect of metformin on cisplatinin human gastric cancer MKN-45 cellline

Objective To evaluate the effect of Metformin and Metformin combined with Cisplatin on human gastric MKN-45 cell line. Methods Treated the human gastric cancer MKN-45 cell line withof the Metformin alone or Metformin combined withCisplatinby different concentrations,thenanalysed the cell survival rate by MTT,checked mRNA levels of survivin, mTOR, and Akt by real-time PCR, and assessed the Cell apoptosis by flow cytometry. Results Metformin alone could inhibit theproliferation and induce apoptosis, but it diminish anti-proliferative effects of theCisplatin when they were used in combination. When MKN-45 cells were treated with Metformin and Cisplatin, the expression of survivin and mTOR were increased. The antagonistic effect of the Metformin on the Cisplatinmight be through survivin, Akt and mTOR signaling pathways. Conclusions Met can inhibit the chemotherapy sensitivity of CispinMKN-45 human gastric cancer cell line.For gastric cancer patients with Type 2 Diabeteswho treated with Cisp,Met should be carefully used.