银杏叶提取物对骨髓间充质干细胞增殖及成骨分化的影响

Effects of GBE on the Proliferation and Osteogenic Differentiation of BMSCs.

Objective: To study the effects of GBE on proliferation and osteogenic differentiation process of rat BMSCs. Methods: By applying different concentrations of GBE on the third-generation of BMSCs,we evaluate its influence on the cell proliferation activity through CCK-8 assay and flow-cytometry analysis. Then we estimated the effect of osteogenic ability of GBE by alizarin red S and alkalinet phosphatase method. RT-PCR was taken to test the expression of the osteogenic specific genes (BMP-2, Runx2) mRNA. Results: There were no statistically significant differences between different concentrations of ginkgo biloba extract in the proliferation on bone marrow mesenchymal stem cells. However, GBE could make the cells in S phase of the cell cycles. GBE could significantly promote the expression of alkaline phosphatase, especially when the concentration was 150 mg/L. The osteogenic specific genes(BMP-2,Runx2)mRNA expression were also relatively higher than control group. Conclusion: Ginkgo biloba extract has a significantly positive effect on the osteogenic differentiation of the BMSCs.