纯镁微弧氧化-HF-硅溶胶复合处理涂层细胞相容性研究

目的:对纯镁微弧氧化经HF-硅溶胶复合处理涂层进行成骨细胞生物相容性检测,评价纯镁表面微弧氧化经不同复合处理涂层后的细胞相容性。方法:实验分为3组,纯镁微弧氧化组为对照组(A组)、纯镁微弧氧化-硅溶胶复合处理组(B组)、纯镁微弧氧化-HF-硅溶胶复合处理组(C组)。将MC3T3-E1系成骨细胞接种在3组材料表面,分别通过扫描电镜、激光共聚焦、CCK-8、ALP对成骨细胞的生长、黏附、增殖以及活性进行检测。结果:扫描电镜和激光共聚焦显示B组和C组表面细胞的生长和黏附均优于A组,C组优于B组。CCK-8和ALP的检测结果显示3组材料表面细胞的增殖、活性为C组＞B组＞A组,3组的比较差异均具有统计学意义。结论:3组材料均具有良好的生物相容性,而纯镁微弧氧化-HF-硅溶胶复合处理后的生物相容性最佳。

Biocompatibility of Pure Magnesium Ultrasonic Micro-arc Oxidation-HF-Silica Sol Compounded Treatment.

Objective: To study the biocompatibility of pure magnesium micro-arc oxidation-HF-silica sol composite treatment, and to evaluate the biocompatibility of pure magnesium micro-arc oxidation after different composite treatment. Methods: The experiment was divided into three groups, the pure magnesium micro-arc oxidation was used as the control group (group A). The pure magnesium micro-arc oxidation-silica sol composite treatment was group (group B). The pure magnesium micro-arc oxidation-HF-silica sol composite treatment was group (group C). MC3T3-E1 osteoblasts were inoculated on material surface of three groups, by scanning electron microscopy (SEM), laser confocal scanning microscope, CCK-8, ALP to test respectively growth, adhesion, proliferation and activity of osteoblasts. Results: The scanning electron microscope and laser confocal scanning microscope demonstrated that on cell growth and adhesion group B and group C surface were superior to group A. Group C was better than that of group B. The results of CCK-8 and ALP detection showed that the proliferation and activity of material surface of three groups were group C > group B > group A. The comparative differences of three groups were statistically significant. Conclusion: The pure magnesium micro-arc oxidation silicon sol composite treatment and pure magnesium micro-arc oxidation-HF-silicon sol composite treatment had all good biocompatibility, and the biocompatibility of HF-silica sol composite treatment was the best.