特发性多发性肌炎外周血淋巴细胞共刺激分子表达的研究

目的观察特发性多发性肌炎患者外周血淋巴细胞共刺激分子CD28、CD152、CD80、CD86表达，进一步分析共刺激分子与疾病的关系。方法研究分三组，即健康对照者（HC，n=5）、特发性多发性肌炎组（IPM，n=8）和肌营养不良组（MD，n=8），后两组患者均经肌肉活检酶组织化学确诊。三组患者均通过流式细胞术检测外周血淋巴细胞CD4、CD8、CD4＋CD28＋、CD8＋CD28＋、CD152、CD80、CD86表达，并计算出CD4＋CD28-、CD8＋CD28-表达。结果与HC组比较，IPM组CD4＋CD28＋表达降低（31.9±6.6 vs 39.5±6.9,P〈0.05），其所占CD4＋T细胞的百分率亦明显下降（92.2%±6.2% vs 98.1%±0.7%,P〈0.05），与之相反的是CD4＋CD28-表达明显升高（2.0±1.8 vs 0.6±0.2,P〈0.05）。IPM组CD4、CD8表达及CD8＋CD28＋/CD8百分比与HC组无明显差异，CD8＋CD28-表达则呈上升趋势。IPM组CD152较HC组明显升高（18.5±13.8 vs 7.1±1.2,P〈0.05），各组间CD80表达无明显差异，CD86以IPM组最高（6.8±1.9 vs 4.6±1.7,P〈0.05）。结论IPM患者外周血CD4阳性的T细胞表面的CD28表达下降，但CD152和CD86表达升高。

Study on the expression of costimulatory molecules in peripheral blood lymphocytes in idiopathic polymyositis

Objective To investigate the expression of CD28, CD152, CD80 and CD86 in peripheral blood lymphoeytes in idiopathic polymyositis, and determine the relationship between these costimu- latory molecules and this disease. Methods Clinical data of healthy controls （HC） （ n = 5）, patients with idiopathic polymyositis （IPM） （ n =8）, muscular dystrophy （MD） （ n =8） were collected. Mus- cular biopsies were carried out on all the patients. The expression of costimulatory molecules CD4＋ CD28＋ , CD8＋ CD28＋ , CD152, CD80 and CD86 in peripheral blood lymphocytes were detected by flow cytometry （FCM）. The expression of CD4＋ CD28- and CD8＋ CD28- were calculated. Results Compared with HC group, the expression of CD4 ＋ CD28 ＋ Tlymphocytes were decreased in IPM group （31.9±6.6 vs 39.5±6. 9, P 〈 0. 05）, and the same pattern was observed in the expression of CD4＋ CD28＋/CD4 （92.2%±6.2% vs 98.%±0. 7%, P 〈 0. 05）. In contrast, the expression of CD4＋ CD28- increased significantly in IPM group compared with HC group （2.0±1. 8 vs 0.6±0.2, P 〈 0.05）. There was no difference in the expression of CD4, CD8 or the percentage of CD8＋ CD28＋/CD8. However, the expression of CD8＋ CD28- had a slight increase. The expression of CD152 in IPM group was higher than in HC group （18.5±13.8 vs 7.1±1.2, P 〈 0. 05）. No differenee in the expression of CD80 was found in the three groups, whereas the expression of CD86 was highest in IPM group （6.8±1.9 vs 4.6± 1.7, P 〈0.05）. Conclusions The expression of CD28 in peripheral blood CD4 positive T lymphoeytes decreased, whereas the expression of CD152 and CD86 increased in idiopathic polymyositis.