基因工程抗角蛋白抗体在正常皮肤和几种表皮增生性皮肤病皮损中的反应定位

目的　探索一株基因工程人源性抗角蛋白Fab抗体在正常皮肤及几种表皮增生性皮肤病皮损中的反应定位。方法　利用从噬菌体抗体库中筛选到的特异表达抗角蛋白Fab片段的质粒转化大肠杆菌 ,IPTG诱导表达出Fab抗体 ,纯化鉴定后用此抗体对正常皮肤及银屑病、鳞癌、基底细胞癌和脂溢性角化病皮损进行免疫组化染色。结果　正常皮肤表皮呈阴性染色 ,毛囊呈阳性染色 ,银屑病、鳞癌、基底细胞癌和脂溢性角化病皮损均呈现明显的阳性着色 ,其中银屑病皮损基底细胞层为强阳性。所有细胞染色部位均位于胞质 ,胞核未见着色 ,真皮为阴性。结论　该株人源性抗角蛋白Fab抗体主要与表皮组织结合 ,对银屑病等表皮增生性皮肤病的损害具有较高特异性。

Immunohistochemical Localization of a Genetically Engineered Human Antibody Against Keratin in Normal Skin and Lesions of some Epidermal Proliferative Diseases

Objective To explore the immunohistochemical localization of a genetically engineered human antibody against keratin in normal skin and lesions of some epidermal proliferative diseases.Methods The specific anti-keratin Fab was previously cloned from a phage antibody library.After being expressed and characterized,the antibody was used to stain the sections of normal skin and lesions from psoriasis,squamous cell carcinoma,basal cell carcinoma and seborrheic keratosis by using immunohistochemistry techniques.Results The staining of negative reaction was observed in normal epidermis.Hair follicle was positive staining.The obvious staining was observed in psoriatic epidermis and lesions of squamous cell carcinoma,basal cell carcinoma and seborrheic keratosis.In addition,the strong positive reaction was observed in the basal cell layers of psoriasis.The binding position of the antibody was in cytoplasm,not in nucleus.Conclusion The immunohistochemical straining of the genetically engineered human antibody against keratin was mainly found in epidermis and was highly specific in lesions of some dermatomes with epidermal proliferation.