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血液病患者血液中肺炎支原体和发酵支原体的检出
引用本文:刘欢乐,胡旭东,周丽萍,谢海啸,王霄霞. 血液病患者血液中肺炎支原体和发酵支原体的检出[J]. 疾病监测, 2009, 24(1): 43-45
作者姓名:刘欢乐  胡旭东  周丽萍  谢海啸  王霄霞
作者单位:浙江省温州医学院第一附属医院,浙江,温州,325000;浙江省温州医学院第一附属医院,浙江,温州,325000;浙江省温州医学院第一附属医院,浙江,温州,325000;浙江省温州医学院第一附属医院,浙江,温州,325000;浙江省温州医学院第一附属医院,浙江,温州,325000
摘    要:目的 探讨血液病患者血液中肺炎支原体和发酵支原体的分离检出。方法 分别对101例确诊为血液病患者和65名非血液病对照者的血液标本进行肺炎和发酵支原体分离培养和聚合酶链反应鉴定,阳性标本进一步经电镜确认。 结果 101例血液病患者血液标本中共计检出肺炎和发酵支原体17例(16.8%),其中肺炎支原体9例(8.9%),发酵支原体8例(7.9%),体检对照组血液中未检出肺炎和发酵支原体(0.0%),组间差异有统计学意义(IP/I0.01)。 结论 血液病患者血液中肺炎支原体和发酵支原体的检出显著高于非血液病对照组,确切机制有待进一步探讨。

关 键 词:肺炎支原体  发酵支原体  血液病  分离培养  聚合酶链反应
收稿时间:2008-06-30

Detection of Mycoplasma pneumonia and Mycoplasma fermentans from the blood of hematopathy patients
LIU Huan-le,HU Xu-dong,ZHOU Li-ping,XIE Hai-xiao,WANG Xiao-xia. Detection of Mycoplasma pneumonia and Mycoplasma fermentans from the blood of hematopathy patients[J]. Disease Surveillance, 2009, 24(1): 43-45
Authors:LIU Huan-le  HU Xu-dong  ZHOU Li-ping  XIE Hai-xiao  WANG Xiao-xia
Affiliation:1.Department of Laboratory Science, The First Affiliate Hospital of Wenzhou Medical College, Wenzhou, Zhejiang 325000, China
Abstract:Objective To discuss the detection of Mycoplasma pneumonia and Mycoplasma fermentans from the blood of hematopathy patients. Methods The blood samples were collected from 101 hematopathy patients and 65 normal volunteers (control group) to isolate M.pneumonia and M.fermentans in Mycoplasma special medium and identified by PCR, and the positive samples were confirmed by electron microscope. Results Seventeen strains (16.8%) of Mycoplasma were detected from 101 blood samples of hematopathy patients, including 9 strains of M.pncumonia and 8 strains of M. fermentans, and no M.pneumonia and M.fermentans were detected from the blood samples of control group. The difference was statistical significant (P〈0.01). Conclusions The detection rate of M.pneumonia and M.fermentans from the blood of hematopathy patients was significant higher than the control group of non-hematopathy patients. The exact mechanism should be explored.
Keywords:Mycoplasmapneumonia  Mycoplasmafermentans  hemopathy  isolation and culture  polymerase chain reaction
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