小鼠树突状细胞介导CT-26实体肿瘤凋亡研究

目的研究负载树突状细胞诱导实体肿瘤凋亡作用,为其用于临床生物治疗提供依据。方法从小鼠骨髓诱导培养树突状细胞,以CT-26肿瘤细胞冻融液为DC负载抗原,测定此负载的DC体内诱导CT-26实体肿瘤凋亡效果。结果诱导的树突状细胞形态典型;CT-26细胞冻融液负载的DC诱导实体瘤外周细胞凋亡明显,而内部细胞罕见凋亡。结论负载DC治疗体积较大的实体瘤作用有限,可能更适合微瘤治疗。

Apoptosis effects of murine dendritic cell on CT-26 solid tumor

Objective: To investigate the apoptosis effects induced with tumor antigen loaded dendritic cells(DCs) and provide evidences for biological therapy based on DC.Methods: The freeze-lysate solution of CT-26 tumor cells was loaded on DC that cultured from BALB/c murine bone marrow,and we detected the effect of apoptosis on solid tumor induced with DCs in vivo.Results: The outside cells of solid tumor could be induced apoptosis with antigen loaded DCs,while inside cells of solid tumor had little apoptosis.Conclusion: The effects of therapy on micro solid tumor with antigen loaded DC are potentially effective but not suitable for large solid tumor.