云南临沧HIV阳性者血液中弓形虫SAG2 基因位点分析

目的 目的 初步了解云南临沧HIV阳性者血液中弓形虫的基因型特征。方法 方法 运用巢式PCR法对弓形虫SAG2基因的两个片段分别进行基因提取、 扩增， 然后对PCR产物进行回收纯化、 酶切及测序， 并与弓形虫基因I型标准株进行对比鉴定。结果 结果 从170份HIV阳性者全血样本中分别扩增出35个弓形虫SAG2基因 （241、 221 bp）， 从其中扩增阳性的全血样本中各选择4份分别进行酶切， 扩增出目的基因片段， 进一步从酶切样本中选择2份与弓形虫基因标准株RH株进行对比， 酶切SAG2基因 （241 bp） 显示血液样本基因型为Ⅰ型或Ⅱ型， 酶切SAG2基因 （221 bp） 确定基因型均为Ⅰ型。结 结论 论 初步确定云南临沧HIV阳性者弓形虫基因型以Ⅰ型为主。

Analysis of sites of Toxoplasma gondii SAG2 gene in blood of HIV-positive people in Lincang City,Yunnan Province

Objective Objective To preliminarily understand the genotype characteristics of Toxoplasma gondii in blood of HIV?posi?tive persons in Lincang City，Yunnan Province. Method Method Two segments of SAG2 gene of T. gondii from blood samples of HIV?positive persons in Lincang City were extracted and amplified by using the nested PCR method and the genotype was identifiedand compared with the standard strain（Type I）of Toxoplasma gondii. Results Results Thirty ? five SAG2 genes（241 bp）and 35SAG2 genes（221 bp）of T. gondii were amplified from 170 blood samples of the HIV?positive people，and 4 of each case wereselected and digested with enzyme，then 2 aim gene fragments of each case were chosen and compared with the standard strain（Type I）of T. gondii. The digestion of SAG2 gene（241 bp）showed the genotype of the blood samples was Type I or Type II，and the digestion of SAG2 gene（221 bp）confirmed that the genotype was Type I. Conclusion Conclusion It is preliminarily confirmedthat the genotype of T. gondii in blood of HIV?positive persons in Lincang City，Yunnan Province is Type I