一种用于前列腺癌细胞体外检测生物导航纳米探针的制备

目的 制备一种用于对人前列腺癌PC3细胞进行体外检测的粒细胞靶向介导的磁性-荧光纳米探针.方法 制备以Fe3O4为核、表面包被siO2和异硫氰酸罗丹明的具有磁性且携带红色荧光的纳米材料,将其与正常人外周血粒细胞按不同比例混合,分别孵育不同时间以检测纳米材料对粒细胞的毒性.选择最佳比例将纳米材料和粒细胞体外结合,得到粒细胞靶向介导的磁性-荧光纳米探针;将PC3细胞与正常人全血细胞分别以不同比例混合,加入上述探针,于荧光显微镜下观察探针的靶向情况.结果 在实验所用的浓度和作用时间范围内,制备的纳米探针对粒细胞存活率无明显影响.镜下可见纳米探针在PC3细胞周围富集,形成“花瓣样”结构,而全血细胞周围无探针富集.结论 本研究制备的磁性-荧光纳米探针不会对粒细胞本身产生明显毒性,利用粒细胞对肿瘤细胞的生物靶向作用可使纳米探针有效地靶向检测肿瘤细胞.

Preparation of a new bio-navigation nanoprobe used for prostate cancer cell detection in vitro

Objective Detect prostate cancer cell in vitro by granulocyte target mediated probe technique which attaches magnetic and fluorescent functions. Methods Material: Prapare the nano material which uses Fe3O4 as the core, SiO2 and isothiocyanate Luo Danming as the shell. To examine the toxicity of nano materials to granulocytes, we mix it with normal human peripheral blood granulocytes in different proportions and co-culture them at different time intervals. Then select the best proportion to combine the two in vitro. Finally we get a granulocyte targeted magnetic fluorescent probe. Next, we mix human prostate cancer cell PC3 and normal human whole blood cells at different proportions, then add the probe, the target situation of the probe is observed under a fluorescence microscope. Results The survival rate of granulocytes was not statistically significant in the concentration range of nano materials used in the experiment(P>0.05). The probes were enriched around the cancer cells and forming a petal like structure under the microscope, but the peripheral blood cells are not enriched by probes. Conclusion The magnetic fluorescent nanomaterials in this study have no toxicity to granulocyte. Using the biological targeting effect of granulocyte on tumor cells, the nano probe can effectively detect tumor cells.