薄层荧光扫描法测定小叶买麻藤等植物中类化合物含量

目的　建立小叶买麻藤中6种类化合物的含量测定方法。方法　在高效硅胶薄层板上，买麻藤丙素、ε-viniferin、白藜芦醇、异丹叶大黄素、银松素以甲苯-冰醋酸-甲醇(3∶1∶0.2)为展开剂，进行二次展开；异丹叶大黄素-3-O-β-D-吡喃葡糖苷以甲苯-乙酸乙酯-冰醋酸-甲醇(1∶2∶0.5∶0.2)为展开剂，一次展开，展开后用液体石蜡-正己烷(1∶1)浸板，荧光扫描法测定。结果　6种化合物的线性关系良好，相关系数在0.9914～0.9999，回收率为94.4%～104.8％，RSD在2.3%～5.1％之间。用此法测定了小叶买麻藤等12种药用植物中此类化合物的含量和分布。结论　为研究和开发含类化合物的药用资源提供了简便、灵敏、准确的含量测定方法。

DETERMINATION OF SIX STILBENES IN GNETUM PARVIFOLIUM (WARB.) C.Y.CHENG BY HPTLC FLUORESCENT SCANNING

AIM　To establish a TLC fluorescent scanning method for separation and determination of the six stilbenes: isorhapotigenin-3-O-β-D-glucopyranoside (I), gnetifolin C (II), ε-viniferin (III), resveratol (IV), isorhapotigenin (V) and pinosylvine (VI) in Gnetum parvifolium. METHODS　The separation of stilbenes II～VI was accomplished on the silica gel plate by twice development using the mobile phase toluene-glacial acetic acid-methanol (3∶1∶0.5). The separation of I was achieved by using the mobile phase toluene-ethyl acetate-glacial acetic acid-methanol(1∶2∶0.5∶0.2). After separation of the stilbenes, the plate was dipped into a mixture of paraffin oil-hexane (1∶1) to protect the fluorescence. The contents of six stilbenes were determined by HPTLC fluorescent scanning with λ_ex=313 nm and λ_em=400 nm (No.2 filter). RESULTS The calibration curves showed good linearity in the range of 17.4～260.7 ng. The recoveries were 96.4%～104.8% with RSD% 2.3%～5.1%. The method has been applied to the analysis of various samples. CONCLUSION　It offered a simple, sensitive, and accurate method for research and utilization resources of stilbenes.