| 不同转染方式对腺病毒介导的基因转染效率的影响 |
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| 引用本文： | 孔霞,郭凌郧,张蕾,郑飞,杨建业,黄永章,唐俊明,王家宁. 不同转染方式对腺病毒介导的基因转染效率的影响[J]. 郧阳医学院学报, 2010, 29(1): 1-4,F0003 |
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| 作者姓名： | 孔霞 郭凌郧 张蕾 郑飞 杨建业 黄永章 唐俊明 王家宁 |
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| 作者单位： | 孔霞,郭凌郧,张蕾,郑飞,杨建业,黄永章,唐俊明(郧阳医学院附属人民医院临床医学研究所,湖北 十堰,442000);王家宁(郧阳医学院附属人民医院临床医学研究所,湖北 十堰 442000;郧阳医学院附属人民医院心内科,湖北 十堰 442000) |
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| 基金项目： | 国家自然科学基金，湖北省卫生厅，湖北省自然基金，湖北省教育厅 |
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| 摘 要： | 目的:研究携带绿色荧光蛋白(Green Fluorescent Protein,GFP)基因的重组腺病毒在大鼠骨髓间充质干细胞(MSCs)中的表达。方法:将纯化的GFP重组腺病毒分别用单次转染和多次转染两种不同方法转染MSC,观察其感染效率和GFP表达水平。结果:培养的MSC呈CD90和CD105强阳性,且具有成骨、成脂肪等多向分化能力;携带绿色荧光蛋白基因的重组腺病毒单次转染和多次转染P3代MSC都呈现出浓度依赖性;单次转染在80MOI时转染效率最高,而多次转染在20MOI时转染效率即已达90%。结论:建立稳定、高效表达Ad-GFP的骨髓间充质干细胞实验体系,为MSC的标记及示踪奠定基础。
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| 关 键 词： | 腺病毒 绿色荧光蛋白 转染 骨髓间充质干细胞 |
Effect of Different Transfection on Gene Transfer Efficiency Mediated with Adenovirus in Mesenchymal Stem Cells |
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| KONG Xia,GUO Ling-yun,ZHANG Lei,ZHENG Fei,YANG Jian-ye,HUANG Yong-zhang,TANG Jun-ming,WANG Jia-ning. Effect of Different Transfection on Gene Transfer Efficiency Mediated with Adenovirus in Mesenchymal Stem Cells[J]. Journal of Yunyang Medical College, 2010, 29(1): 1-4,F0003 |
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| Authors: | KONG Xia GUO Ling-yun ZHANG Lei ZHENG Fei YANG Jian-ye HUANG Yong-zhang TANG Jun-ming WANG Jia-ning |
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| Affiliation: | KONG Xia1,GUO Ling-yun1,ZHANG Lei1,ZHENG Fei1,YANG Jian-ye1,HUANG Yong-zhang1,TANG Jun-ming1,WANG Jia-ning1,2 (1Institute of Clinical Medicine,2Department of Cardiology,Renmin Hospital,Yunyang Medical College,Shiyan,Hubei 442000,China) |
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| Abstract: | Objective To investigate the expression of recombinant adenovirus encoding green fluorescent protein(GFP) in mesenchymal stem cells(MSCs).Methods The purified recombinant adenovirus encoding GFP(Ad-GFP) was transfected into MSCs with single or multiple infection,the transfer efficiency of Ad-GFP and the expression level of GFP were measured.Results Cultured MSCs were observed with a multiple differentiation potential into osteoblast,lipoblast and highly expressed CD105 and CD90.The Ad-GFP transfection of P3... |
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| Keywords: | Adenovirus Green fluorescent protein Transfection Mesenchymal stem cells |
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