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Hepatoprotective potential of Tecomella undulata stem bark is partially due to the presence of betulinic acid
Authors:Mahendra Jain  Rakhee Kapadia  Ravirajsinh N. Jadeja  Menaka C. Thounaojam  Ranjitsinh V. Devkar  S.H. Mishra
Affiliation:1. Pharmacognosy Laboratory, Faculty of Pharmaceutical Sciences, Shoolini University, Solan 173212, Himachal Pradesh, India;2. Herbal Drug Technology Laboratory, Pharmacy Department, Faculty of Technology and Engineering, The M.S. University of Baroda, Kalabhavan, Vadodara 390001, Gujarat, India;3. Novel Drug Delivery System Laboratory, Pharmacy Department, Faculty of Technology and Engineering, The M.S. University of Baroda, Kalabhavan, Vadodara 390001, Gujarat, India;4. Division of Phytothrapeutics and Metabolic Endocrinology, Department of Zoology, Faculty of Science, The M.S. University of Baroda, Gujarat, India
Abstract:

Ethnopharmacological relevance

Tecomella undulata (TU;` Family Bignoniaceae) is used in Indian Ayurvedic system of medicine for treating various diseases including hepatic ailments. It is also incorporated in various marketed hepatoprotective polyherbal formulations.

Aim

The present study was aimed at evaluating possible hepatoprotective role of isolated compounds from TU stem bark (TSB) using in vitro and in vivo experimental models.

Methods

In vitro cytotoxicity and hepatoprotective potential of various extract, fractions and isolated compounds from TU stem bark were evaluated using HepG2 cells. Rats were pre-treated with TU methanolic extract (TSB-7) or betulinic acid (MS-2) or silymarin for 7 days followed by a single dose of CCl4 (0.5 ml/kg, i.p.). Plasma markers of hepatic damage, hepatic antioxidants and indices of lipid peroxidation along with microscopic evaluation of liver were assessed in control and treatment groups.

Results

TSB-2 and MS-1 accounted for significant cell death whereas; TSB-1, TBS-7, TSB-9, TSB-10 and, MS-2 did not register significant cytotoxicity. Further, non-cytotoxic components exhibited ascending grade of hepatoprotection in vitro (TSB-104 treated rats prevented hepatocyte damage as evidenced by biochemical and histopathological observations.

Conclusion

It can be concluded that, hepatoprotective potential of Tecomella undulata stem bark is partially due to the presence of betulinic acid.
Keywords:AST, Aspartate amino transferase   ALT, Alanine amino transferase   CCL4, Carbon tetrachloride   BW, Bodyweight   w/w, Weight/weight   EDTA, Ethylene diamine tetra acetic acid   TLC, Thin layer chromatography   MTT, 3-(4,5-Dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide   OECD, Organisation for Economic Co-operation and Development   ALP, Alkaline phosphatase   SOD, Superoxide dismutase   CAT, Catalase   GSH, Reduced glutathione   LPO, Lipid peroxidation   MDA, Malonaldehyde   AA, Ascorbic acid   ROS, Reactive oxygen species.
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