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| 中国大陆首例人感染高致病性禽流感病毒(H5N1)病例的实验室诊断 | |
| 引用本文： | 董婕,张红,刘运芝,张烨,王敏,余宏杰,杨维中,徐翠玲,向妮娟,李中杰,陈裕旭,郭元吉,李德新,李俊华,王宇,舒跃龙.中国大陆首例人感染高致病性禽流感病毒(H5N1)病例的实验室诊断[J].中华实验和临床病毒学杂志,2006,20(2):14-16. |
| 作者姓名： | 董婕 张红 刘运芝 张烨 王敏 余宏杰 杨维中 徐翠玲 向妮娟 李中杰 陈裕旭 郭元吉 李德新 李俊华 王宇 舒跃龙 |
| 作者单位： | 1. 100052,北京,中国疾病预防控制中心病毒病所,传染病预防控制国家重点实验室 2. 湖南省疾病预防控制中心 3. 中国疾病预防控制中心 |
| 基金项目： | 国家自然科学基金资助（30599433）.感谢湖南省卫生厅、湘潭市疾病预防控制中心、湘潭县疾病预防控制中心、湘潭市妇幼保健院和湖南省儿童医院对本工作的大力支持和协助. |
| 摘 要： | 目的 对2005年10月湖南省湘潭市湘潭县发生的一名不明原因肺炎病例进行实验室检测,以确定导致该病例的主要病因.方法 采集病例呼吸道标本以及血清标本,对呼吸道标本利用分子鉴别诊断技术以及RT-PCR和实时荧光定量RT-PCR检测病毒核酸;通过血凝抑制试验以及微量中和试验检测血清中的特异性抗体.结果 该病例所有的呼吸道标本H5N1病毒特异性核酸及病毒分离均为阴性.红细胞凝集抑制及微量中和实验显示,恢复期血清较急性期血清H5N1特异性抗体阳转并且有4倍以上增高.结论 通过实验室检测结果分析,该病例为中国大陆第一例人感染高致病性禽流感病毒（H5N1）实验室确诊病例. |
| 关 键 词： | 禽流感 H5N1亚型 不明原因肺炎 实验室检测 |
| 收稿时间： | 2006-03-12 |
| 修稿时间： | 2006年3月12日 |
Laboratory diagnoses of the first confirmed human case of avian influenza A (H5N1) in the mainland of China |
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| DONG Jie,ZHANG Hong,LIU Yun-zhi,ZHANG Ye,WANG Min,YU Hong-jie,YANG Wei-zhong,XU Cui-ling,XIANG Ni-juan,LI Zhong-jie,CHEN Yu-xu,GUO Yuan-ji,LI De-xin,LI Jun-hua,WANG Yu,SHU Yue-long.Laboratory diagnoses of the first confirmed human case of avian influenza A (H5N1) in the mainland of China[J].Chinese Journal of Experimental and Clinical Virology,2006,20(2):14-16. | |
| Authors: | DONG Jie ZHANG Hong LIU Yun-zhi ZHANG Ye WANG Min YU Hong-jie YANG Wei-zhong XU Cui-ling XIANG Ni-juan LI Zhong-jie CHEN Yu-xu GUO Yuan-ji LI De-xin LI Jun-hua WANG Yu SHU Yue-long |
| Institution: | Institute for Viral Disease Control and Prevention Chinese Center for Disease Control and Prevention, State Key Laboratory for Infectious Disease Control and Prevention, Beijing 100052, China |
| Abstract: | Objective To determine the etiologic agent of an atypical pneumonia human case admitted to Xiangtan City hospital,Hunan province in Oct.2005. Methods The patient's respiratory tract samples and serum were collected. Throat swabs were tested with MDD (Molecular Differential Diagnostic Technology),RT-PCR and Real-Time RT-PCR. The serum specimens were tested by microneutralization and hemagglutination-inhibition asssays. Results The results of nucleic acid detection of all respiratory samples were negative and virus isolation was also negative. The H5-specific antibodies of convalescence showed a 4-fold greater rise than acute phase. Conclusion The atypical pneumonias case was confirmed as the first human case of avian influenza A (H5N1) infection in the mainland of China. |
| Keywords: | Avian influenza HSN1 subtype Atypical pneumonia case Laboratory diagnosis |
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