| 雷公藤内酯醇对神经胶质瘤U87细胞侵袭性抑制的体外研究 |
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| 引用本文： | 李建斌,翟玉平,祝新根,吕世刚,刘峰,程祖珏.雷公藤内酯醇对神经胶质瘤U87细胞侵袭性抑制的体外研究[J].分子诊断与治疗杂志,2013,0(3):181-184. |
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| 作者姓名： | 李建斌 翟玉平 祝新根 吕世刚 刘峰 程祖珏 |
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| 作者单位： | 1. 南昌大学第二附属医院神经外科,江西,南昌330006
2. 湖口县人民医院神经外科,江西,九江332500 |
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| 基金项目： | 江西省教育厅项目（EF130） |
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| 摘 要： | 目的 研究雷公藤内酯醇（triptolide）对体外培养的胶质瘤U87细胞侵袭性的抑制作用,并探讨其作用分子机制. 方法 使用MTT法检测雷公藤内酯醇对胶质瘤U87细胞增殖抑制作用, Transwell实验检测雷公藤内酯醇处理后细胞侵袭能力的变化,进一步通过Real-Time PCR和Western印迹法检测雷公藤内酯醇对U87胶质瘤细胞的组织蛋白酶B（Cathepsins B,CB）、基质金属蛋白酶9（MMP-9）表达的影响. 结果 雷公藤内酯醇以剂量-效应方式抑制胶质瘤细胞增殖,同时可减弱U87胶质瘤细胞的侵袭能力,下调其CB、MMP-9表达水平. 结论 在体外实验中雷公藤内酯醇抑制U87胶质瘤细胞侵袭性生长的机制与下调CB及MMP-9的表达,降低胶质瘤细胞水解细胞外基质的能力有关.
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| 关 键 词： | 雷公藤内酯醇 胶质瘤 侵袭 |
The anti-invation effect of triptolide for U87 glioma in vitro |
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| Institution: | LI Jianbin, ZAI Yuping, ZHU Xingen, LV Shigang, LIU Feng, CHENG Zhujue(1.Department of Neurosurgery, The Second Affiliated Hospital of Nanchang University, Jiangxi, Nanchang 330006, China. 2. Department ofNeurosurgery, Hukou Hospital, Jiangxi, Jiujiang 332500, China) |
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| Abstract: | Objective To study the anti-invation effect of triptolide for U87 glioma cells and its molecular mechanism. Methods MTT assay was used to determine the growth inhibition by triptolide in glioma U87 cells. Tumor invasion and migration were examined by Transwell Chamber assay. Real-Time PCR and Westerm blot was conducted to detect including the alteration ofCathepsins B （CB） and MMP-9. Results Triptolide could significantly inhibite glioma U87 cell proliferation, reduce the cell ability of invasion, down- regulating MMP-9 and CB expression level. Conclusion Triptolide could depress the invasion ofglioma cells owing to down-regulating the expression level of MMP-9 and CB, reduce the ability of hydrolyzing extracellular matrix in vitro. |
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| Keywords: | Triptolide Glioma Invasion |
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