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| 人胱抑素C单克隆抗体的制备、鉴定及初步应用 | |
| 引用本文： | 陈婷梅,俸家富,涂植光. 人胱抑素C单克隆抗体的制备、鉴定及初步应用[J]. 中国免疫学杂志, 2008, 24(9) |
| 作者姓名： | 陈婷梅 俸家富 涂植光 |
| 作者单位： | 重庆医科大学检验系,临床检验诊断学省部共建教育部重点实验室,重庆市临床检验诊断学重点实验室,重庆,400016 |
| 摘 要： | 目的:制备人胱抑素C(Cystatin C,Cys C)的单克隆抗体(McAb),并建立检测人血清Cys C的颗粒增强透射免疫浊度分析法(PETLA).方法:构建人Cys C的原核表达载体pET32a( )/Cys C,纯化Cys C重组蛋白并免疫BALB/c小鼠,杂交瘤技术制备Cys C的McAb;以自制的McAb包被乳胶颗粒,建立PETIA法测定血清Cys C,并对其分析性能进行初步方法学评价.结果:建立分泌抗Cys C单克隆抗体的杂交瘤细胞株3株,其分泌的McAb为IgG1,Western blot检测证实该抗体能与目的蛋白发生特异性结合;将杂交瘤细胞注射于小鼠腹腔,腹水中Cys C McAb效价为1∶4×106;用自制的McAb建立定量测定Cys C的PETIA,方法学评价试验结果证实我们建立的PETIA法,与进口试剂有很好的可比性,具有较满意的方法学性能.结论:成功制备了抗Cys C单克隆抗体,该抗体可用于建立定量检测Cys C的PETIA法. |
| 关 键 词： | 胱抑素C 单克隆抗体 颗粒增强透射免疫分析法 |
Preparation,characterization and preliminary application of monoclonal antibody against cystatin C |
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| CHEN Ting-Mei,FENG Jia-Fu,TU Zhi-Guang. Preparation,characterization and preliminary application of monoclonal antibody against cystatin C[J]. Chinese Journal of Immunology, 2008, 24(9) | |
| Authors: | CHEN Ting-Mei FENG Jia-Fu TU Zhi-Guang |
| Affiliation: | CHEN Ting-Mei,FENG Jia-Fu,TU Zhi-Guang.The Key Laboratory of Clinical Diagnosis of Education Ministry,Faculty of Laboratory Medicine,Chongqing Medical University,Chongqing 400016,China |
| Abstract: | Objective:To prepare monoclonal antibodies(McAb)against cystatin C(Cys C)and to establish the particle enhanced turbidimetric immunoassay(PETIA)for determining human serum Cys C.Methods:The prokaryotic expression vector pET32a(+)/Cys C was constructed and Cys C expression was induced.McAbs against Cys C were prepared with the hybridoma technique after mice were immunized with the purified recombinant protein.Then the McAbs were covalently attached to uniform microparticles,PETIA method for determination of ... |
| Keywords: | Cystatin C Monoclonal antibody PETIA |
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